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5jjn

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Revision as of 07:52, 10 June 2020

Structure of the SRII/HtrII(G83F) Complex in P212121 space group ("V" shape)

Structural highlights

5jjn is a 4 chain structure with sequence from Atcc 35678. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Gene:	sop2, sopII (ATCC 35678), htr2, htrII (ATCC 35678)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BACS2_NATPH] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light. [HTR2_NATPH] Transduces signals from the phototaxis receptor sensory rhodopsin II (SR-II) to the flagellar motor. Responds to light changes through the variation of the level of methylation. Also acts as a chemotransducer.

Publication Abstract from PubMed

The complex of two membrane proteins, sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII), mediates negative phototaxis in halobacteria N. pharaonis. Upon light activation NpSRII triggers a signal transduction chain homologous to the two-component system in eubacterial chemotaxis. Here we report on crystal structures of the ground and active M-state of the complex in the space group I212121. We demonstrate that the relative orientation of symmetrical parts of the dimer is parallel ("U"-shaped) contrary to the gusset-like ("V"-shaped) form of the previously reported structures of the NpSRII/NpHtrII complex in the space group P21212, although the structures of the monomers taken individually are nearly the same. Computer modeling of the HAMP domain in the obtained "V"- and "U"-shaped structures revealed that only the "U"-shaped conformation allows for tight interactions of the receptor with the HAMP domain. This is in line with existing data and supports biological relevance of the "U" shape in the ground state. We suggest that the "V"-shaped structure may correspond to the active state of the complex and transition from the "U" to the "V"-shape of the receptor-transducer complex can be involved in signal transduction from the receptor to the signaling domain of NpHtrII.

New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex.,Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Remeeva A, Polovinkin V, Utrobin P, Balandin T, Engelhard M, Buldt G, Gordeliy V Sci Rep. 2017 Feb 6;7:41811. doi: 10.1038/srep41811. PMID:28165484^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Remeeva A, Polovinkin V, Utrobin P, Balandin T, Engelhard M, Buldt G, Gordeliy V. New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex. Sci Rep. 2017 Feb 6;7:41811. doi: 10.1038/srep41811. PMID:28165484 doi:http://dx.doi.org/10.1038/srep41811

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5jjn"

@@ Line 1: / Line 1: @@
 ==Structure of the SRII/HtrII(G83F) Complex in P212121 space group ("V" shape)==
-<StructureSection load='5jjn' size='340' side='right' caption='[[5jjn]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='5jjn' size='340' side='right'caption='[[5jjn]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5jjn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JJN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5jjn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35678 Atcc 35678]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JJN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JJN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jjn OCA], [http://pdbe.org/5jjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jjn RCSB], [http://www.ebi.ac.uk/pdbsum/5jjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jjn ProSAT]</span></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sop2, sopII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 ATCC 35678]), htr2, htrII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 ATCC 35678])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jjn OCA], [http://pdbe.org/5jjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jjn RCSB], [http://www.ebi.ac.uk/pdbsum/5jjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jjn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 5jjn" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
+*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Atcc 35678]]
+[[Category: Large Structures]]
 [[Category: Balandin, T]]
 [[Category: Borshchevskiy, V]]

5jjn

From Proteopedia

Revision as of 07:52, 10 June 2020

Structure of the SRII/HtrII(G83F) Complex in P212121 space group ("V" shape)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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