1te6

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[[Image:1te6.gif|left|200px]]
[[Image:1te6.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1te6 |SIZE=350|CAPTION= <scene name='initialview01'>1te6</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1te6", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1te6| PDB=1te6 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1te6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1te6 OCA], [http://www.ebi.ac.uk/pdbsum/1te6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1te6 RCSB]</span>
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}}
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'''Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom'''
'''Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom'''
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[[Category: Lovelace, L.]]
[[Category: Lovelace, L.]]
[[Category: Minor, W.]]
[[Category: Minor, W.]]
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[[Category: enolase]]
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[[Category: Enolase]]
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[[Category: isozyme]]
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[[Category: Isozyme]]
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[[Category: negative cooperativity]]
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[[Category: Negative cooperativity]]
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[[Category: neuron]]
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[[Category: Neuron]]
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[[Category: surface charge]]
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[[Category: Surface charge]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:50:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:54:49 2008''
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Revision as of 06:50, 3 May 2008

Image:1te6.gif

Template:STRUCTURE 1te6

Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom


Overview

Human neuron-specific enolase (NSE) or isozyme gamma has been expressed with a C-terminal His-tag in Escherichia coli. The enzyme has been purified, crystallized and its crystal structure determined. In the crystals the enzyme forms the asymmetric complex NSE x Mg2 x SO4/NSE x Mg x Cl, where "/" separates the dimer subunits. The subunit that contains the sulfate (or phosphate) ion and two magnesium ions is in the closed conformation observed in enolase complexes with the substrate or its analogues; the other subunit is in the open conformation observed in enolase subunits without bound substrate or analogues. This indicates negative cooperativity for ligand binding between subunits. Electrostatic charge differences between isozymes alpha and gamma, -19 at physiological pH, are concentrated in the regions of the molecular surface that are negatively charged in alpha, i.e. surface areas negatively charged in alpha are more negatively charged in gamma, while areas that are neutral or positively charged tend to be charge-conserved.

About this Structure

1TE6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase., Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L, J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:15289101 Page seeded by OCA on Sat May 3 09:50:48 2008

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