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5un0

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Revision as of 14:35, 6 November 2017

Crystal Structure of Mycobacterium Tuberculosis Proteasome-assembly chaperone homologue Rv2125

Structural highlights

5un0 is a 3 chain structure with sequence from "bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	ERS124361_03483, RN05_2270 ("Bacillus tuberculosis" (Zopf 1883) Klein 1884)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

A previous bioinformatics analysis identified the Mycobacterium tuberculosis (M. tuberculosis) proteins Rv2125 and Rv2714 as orthologs of the eukaryotic proteasome assembly chaperone 2 (PAC2). We set out to investigate whether Rv2125 or Rv2714 could function in proteasome assembly. We solved the crystal structure of Rv2125 at 3.0 A resolution, which showed an overall fold similar to that of the PAC2 family proteins that include the archaeal PbaB and the yeast Pba1. However, Rv2125 and Rv2714 formed trimers, whereas PbaB forms tetramers and Pba1 dimerizes with Pba2. We also found that purified Rv2125 and Rv2714 could not bind to M. tuberculosis 20S core particles. Finally, proteomic analysis showed that the levels of known proteasome component and substrate proteins were not affected by disruption of Rv2125 in M. tuberculosis Our work suggests that Rv2125 does not participate in bacterial proteasome assembly or function.Importance Although many bacteria do not encode proteasomes, M. tuberculosis not only uses proteasomes, it has also evolved a post-translational modification system called pupylation to deliver proteins to the proteasome. Proteasomes are essential for M. tuberculosis to cause lethal infections in animals, thus determining how proteasomes are assembled may help identify new ways to combat tuberculosis. We solved the structure of a predicted proteasome assembly factor, Rv2125, and isolated a genetic mutant of Rv2125 in M. tuberculosis Our structural, biochemical, and genetic studies indicate that Rv2125 and Rv2714 do not function as proteasome assembly chaperones and are unlikely to have roles in proteasome biology in mycobacteria.

Structural analysis of Mycobacterium tuberculosis homologues of the eukaryotic proteasome assembly chaperone 2 (PAC2).,Bai L, Jastrab JB, Isasa M, Hu K, Yu H, Gygi SP, Darwin KH, Li H J Bacteriol. 2017 Feb 13. pii: JB.00846-16. doi: 10.1128/JB.00846-16. PMID:28193903^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bai L, Jastrab JB, Isasa M, Hu K, Yu H, Gygi SP, Darwin KH, Li H. Structural analysis of Mycobacterium tuberculosis homologues of the eukaryotic proteasome assembly chaperone 2 (PAC2). J Bacteriol. 2017 Feb 13. pii: JB.00846-16. doi: 10.1128/JB.00846-16. PMID:28193903 doi:http://dx.doi.org/10.1128/JB.00846-16

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5un0"

@@ Line 3: / Line 3: @@
 <StructureSection load='5un0' size='340' side='right' caption='[[5un0]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5un0]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UN0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5un0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UN0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UN0 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5un0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5un0 OCA], [http://pdbe.org/5un0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5un0 RCSB], [http://www.ebi.ac.uk/pdbsum/5un0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5un0 ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERS124361_03483, RN05_2270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5un0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5un0 OCA], [http://pdbe.org/5un0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5un0 RCSB], [http://www.ebi.ac.uk/pdbsum/5un0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5un0 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

5un0

From Proteopedia

Revision as of 14:35, 6 November 2017

Crystal Structure of Mycobacterium Tuberculosis Proteasome-assembly chaperone homologue Rv2125

Structural highlights

Publication Abstract from PubMed

References

Contents

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