1tkv
From Proteopedia
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[[Image:1tkv.jpg|left|200px]] | [[Image:1tkv.jpg|left|200px]] | ||
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'''Solution Structure of T4 AsiA Dimer''' | '''Solution Structure of T4 AsiA Dimer''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. This structure supersedes the now removed PDB entry | + | 1TKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ka3 1ka3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Schirf, V.]] | [[Category: Schirf, V.]] | ||
[[Category: Werner, M H.]] | [[Category: Werner, M H.]] | ||
| - | [[Category: | + | [[Category: Anti-sigma]] |
| - | [[Category: | + | [[Category: Homodimer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:04:29 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:04, 3 May 2008
Solution Structure of T4 AsiA Dimer
Overview
The bacteriophage T4 AsiA protein is a multifunctional protein that simultaneously acts as both a repressor and activator of gene expression during the phage life cycle. These dual roles with opposing transcriptional consequences are achieved by modification of the host RNA polymerase in which AsiA binds to conserved region 4 (SR4) of sigma(70), altering the pathway of promoter selection by the holoenzyme. The mechanism by which AsiA flips this genetic switch has now been revealed, in part, from the three-dimensional structure of AsiA and the elucidation of its interaction with SR4. The structure of AsiA is that of a novel homodimer in which each monomer is constructed as a seven-helix bundle arranged in four overlapping helix-loop-helix elements. Identification of the protein interfaces for both the AsiA homodimer and the AsiA-sigma(70) complex reveals that these interfaces are coincident. Thus, the AsiA interaction with sigma(70) necessitates that the AsiA homodimer dissociate to form an AsiA-SR4 heterodimer, exchanging one protein subunit for another to alter promoter choice by RNA polymerase.
About this Structure
1TKV is a Single protein structure of sequence from Enterobacteria phage t4. This structure supersedes the now removed PDB entry 1ka3. Full crystallographic information is available from OCA.
Reference
Flipping a genetic switch by subunit exchange., Lambert LJ, Schirf V, Demeler B, Cadene M, Werner MH, EMBO J. 2001 Dec 17;20(24):7149-59. PMID:11742991 Page seeded by OCA on Sat May 3 10:04:29 2008
