5v3i
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the VS ribozyme - wild-type C634== | |
| + | <StructureSection load='5v3i' size='340' side='right' caption='[[5v3i]], [[Resolution|resolution]] 3.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5v3i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V3I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V3I FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v3i OCA], [http://pdbe.org/5v3i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v3i RCSB], [http://www.ebi.ac.uk/pdbsum/5v3i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v3i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Varkud satellite (VS) ribozyme catalyzes site-specific RNA cleavage and ligation reactions. Recognition of the substrate involves a kissing loop interaction between the substrate and the catalytic domain of the ribozyme, resulting in a rearrangement of the substrate helix register into a so-called "shifted" conformation that is critical for substrate binding and activation. We report a 3.3 A crystal structure of the complete ribozyme that reveals the active, shifted conformation of the substrate, docked into the catalytic domain of the ribozyme. Comparison to previous NMR structures of isolated, inactive substrates provides a physical description of substrate remodeling, and implicates roles for tertiary interactions in catalytic activation of the cleavage loop. Similarities to the hairpin ribozyme cleavage loop activation suggest general strategies to enhance fidelity in RNA folding and ribozyme cleavage. | ||
| - | + | Structural Basis for Substrate Helix Remodeling and Cleavage Loop Activation in the Varkud Satellite Ribozyme.,DasGupta S, Suslov NB, Piccirilli JA J Am Chem Soc. 2017 Jul 19;139(28):9591-9597. doi: 10.1021/jacs.7b03655. Epub, 2017 Jul 3. PMID:28625058<ref>PMID:28625058</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Piccirilli, J | + | <div class="pdbe-citations 5v3i" style="background-color:#fffaf0;"></div> |
| - | [[Category: Suslov, N | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: DasGupta, S]] | ||
| + | [[Category: Piccirilli, J A]] | ||
| + | [[Category: Suslov, N B]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Shifted substrate]] | ||
Revision as of 04:08, 4 August 2017
Crystal structure of the VS ribozyme - wild-type C634
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