5wxm

From Proteopedia

(Difference between revisions)

Revision as of 11:26, 6 November 2017

Crystal structure of the Imp3 and Mpp10 complex

Structural highlights

5wxm is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Gene:	IMP3, YHR148W (Baker's yeast), MPP10, YJR002W, J1411, YJR83.5 (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IMP3_YEAST] Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing.^[1] ^[2] [MPP10_YEAST] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing.^[3] ^[4]

Publication Abstract from PubMed

Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.

Molecular architecture of the 90S small subunit pre-ribosome.,Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee SJ, Baserga SJ. Imp3p and Imp4p, two specific components of the U3 small nucleolar ribonucleoprotein that are essential for pre-18S rRNA processing. Mol Cell Biol. 1999 Aug;19(8):5441-52. PMID:10409734
↑ Gerczei T, Correll CC. Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA. Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15301-6. Epub 2004 Oct 15. PMID:15489263 doi:http://dx.doi.org/10.1073/pnas.0406819101
↑ Gerczei T, Correll CC. Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA. Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15301-6. Epub 2004 Oct 15. PMID:15489263 doi:http://dx.doi.org/10.1073/pnas.0406819101
↑ Dunbar DA, Wormsley S, Agentis TM, Baserga SJ. Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast. Mol Cell Biol. 1997 Oct;17(10):5803-12. PMID:9315638
↑ Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K. Molecular architecture of the 90S small subunit pre-ribosome. Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370 doi:http://dx.doi.org/10.7554/eLife.22086

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wxm"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5wxm is ON HOLD  until Jan 07 2019
+==Crystal structure of the Imp3 and Mpp10 complex==
+<StructureSection load='5wxm' size='340' side='right' caption='[[5wxm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5wxm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WXM FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IMP3, YHR148W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), MPP10, YJR002W, J1411, YJR83.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wxm OCA], [http://pdbe.org/5wxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wxm RCSB], [http://www.ebi.ac.uk/pdbsum/5wxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wxm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/IMP3_YEAST IMP3_YEAST]] Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing.<ref>PMID:10409734</ref> <ref>PMID:15489263</ref>  [[http://www.uniprot.org/uniprot/MPP10_YEAST MPP10_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA. Required for the early cleavages at sites A0, A1 and A2 during 18S ribosomal pre-RNA processing.<ref>PMID:15489263</ref> <ref>PMID:9315638</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of Saccharomyces cerevisiae 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.
-Authors: Ye, K., Zheng, S.
+Molecular architecture of the 90S small subunit pre-ribosome.,Sun Q, Zhu X, Qi J, An W, Lan P, Tan D, Chen R, Wang B, Zheng S, Zhang C, Chen X, Zhang W, Chen J, Dong MQ, Ye K Elife. 2017 Feb 28;6. pii: e22086. doi: 10.7554/eLife.22086. PMID:28244370<ref>PMID:28244370</ref>
-Description: Crystal structure of the Imp3 and Mpp10 complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5wxm" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Baker's yeast]]
 [[Category: Ye, K]]
 [[Category: Zheng, S]]
+[[Category: Components of 90s preribosome]]
+[[Category: Protein complex]]
+[[Category: Ribosomal protein]]

5wxm

From Proteopedia

Revision as of 11:26, 6 November 2017

Crystal structure of the Imp3 and Mpp10 complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox