2oar

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2oar" [edit=sysop:move=sysop])
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2oar.png|left|200px]]
 
==Mechanosensitive Channel of Large Conductance (MscL)==
==Mechanosensitive Channel of Large Conductance (MscL)==
 +
<StructureSection load='2oar' size='340' side='right' caption='[[2oar]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycta Mycta]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OAR FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [http://pdbe.org/2oar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [http://www.ebi.ac.uk/pdbsum/2oar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[[http://www.uniprot.org/uniprot/MSCL_MYCTU MSCL_MYCTU]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oar_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oar ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha helices and a third cytoplasmic alpha helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.
-
===Model of Channel Opening===
+
Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.,Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC Science. 1998 Dec 18;282(5397):2220-6. PMID:9856938<ref>PMID:9856938</ref>
-
<applet load='1kyk' size='300' frame='true' align='right' caption='Theoretical Models'
+
-
scene='Goodsell_Sandbox/1kyl/1' />
+
-
Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> of the <scene name='Goodsell_Sandbox/1kyk/1'>closed</scene> (initial scene), <scene name='Goodsell_Sandbox/1kyl/1'>intermediate</scene>, and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.
+
-
{{Clear}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
----
+
</div>
-
<br>
+
<div class="pdbe-citations 2oar" style="background-color:#fffaf0;"></div>
-
<!--
+
-
The FIRST line below this paragraph, containing "STRUCTURE_2oar", creates the "Structure Box" on the page.
+
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
-
or leave the SCENE parameter empty for the default display.
+
-
 
+
-
The SECOND line below this paragraph, {{ABSTRACT_PUBMED_9856938}}, adds the Publication Abstract to the page
+
-
(as it appears on PubMed at http://www.pubmed.gov), where 9856938 is the PubMed ID number.
+
-
-->
+
-
{{STRUCTURE_2oar| PDB=2oar | SCENE= }}
+
-
{{ABSTRACT_PUBMED_9856938}}
+
-
 
+
-
==About this Structure==
+
-
2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]</ref> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].
+
==See Also==
==See Also==
-
Many structural features of a related channel, MscS, including animated morphs of opening an closing, are illustrated at [[Mechanosensitive channels: opening and closing]].
+
*[[Ion channels|Ion channels]]
-
 
+
*[[Mechanosensitive channels: opening and closing|Mechanosensitive channels: opening and closing]]
-
==References and Notes==
+
== References ==
<references/>
<references/>
-
[[Category: Mycobacterium tuberculosis]]
+
__TOC__
-
[[Category: Single protein]]
+
</StructureSection>
-
[[Category: Chang, G.]]
+
[[Category: Mechanosensitive Channels]]
-
[[Category: Lee, A T.]]
+
[[Category: Mycta]]
-
[[Category: Rees, D C.]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Spencer, R H.]]
+
[[Category: Chang, G]]
-
[[Category: Steinbacher, S.]]
+
[[Category: Lee, A T]]
-
[[Category: Strop, P.]]
+
[[Category: Rees, D C]]
 +
[[Category: Spencer, R H]]
 +
[[Category: Steinbacher, S]]
 +
[[Category: Strop, P]]
 +
[[Category: Membrane protein]]
[[Category: Stretch activated ion channel mechanosensitive]]
[[Category: Stretch activated ion channel mechanosensitive]]
- 
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 20 12:12:19 2008''
 

Revision as of 09:23, 26 December 2018

Mechanosensitive Channel of Large Conductance (MscL)

2oar, resolution 3.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA, Eric Martz, David S. Goodsell, Eran Hodis

Retrieved from "http://52.214.119.220/wiki/index.php/2oar"
Personal tools