1tvm
From Proteopedia
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[[Image:1tvm.gif|left|200px]] | [[Image:1tvm.gif|left|200px]] | ||
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'''NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system''' | '''NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system''' | ||
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NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963640 16963640] | NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963640 16963640] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
| - | [[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]] | [[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]] | ||
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[[Category: Volpon, L.]] | [[Category: Volpon, L.]] | ||
[[Category: Young, C R.]] | [[Category: Young, C R.]] | ||
| - | [[Category: | + | [[Category: Bsgi]] |
| - | [[Category: | + | [[Category: E. coli]] |
| - | [[Category: | + | [[Category: Four-stranded parallel open twisted beta sheet flanked by alpha helices on both side]] |
| - | [[Category: | + | [[Category: Montreal-kingston bacterial structural genomics initiative]] |
| - | [[Category: | + | [[Category: P-loop]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:25:24 2008'' | |
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Revision as of 07:25, 3 May 2008
NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system
Overview
The phosphoenolpyruvate-dependent carbohydrate transport system (PTS) couples uptake with phosphorylation of a variety of carbohydrates in prokaryotes. In this multienzyme complex, the enzyme II (EII), a carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. Among the five families of EIIs identified in Escherichia coli, the galactitol-specific transporter (II(gat)) belongs to the glucitol family and is structurally the least well-characterized. Here, we used nuclear magnetic resonance (NMR) spectroscopy to solve the three-dimensional structure of the IIB subunit (GatB). GatB consists of a central four-stranded parallel beta-sheet flanked by alpha-helices on both sides; the active site cysteine of GatB is located at the beginning of an unstructured loop between beta1 and alpha1 that folds into a P-loop-like structure. This structural arrangement shows similarities with other IIB subunits but also with mammalian low molecular weight protein tyrosine phosphatases (LMW PTPase) and arsenate reductase (ArsC). An NMR titration was performed to identify the GatA-interacting residues.
About this Structure
1TVM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:16963640 Page seeded by OCA on Sat May 3 10:25:24 2008
Categories: Escherichia coli | Single protein | BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative. | Cygler, M. | Gehring, K. | Iannuzzi, P. | Lim, N S. | Volpon, L. | Young, C R. | Bsgi | E. coli | Four-stranded parallel open twisted beta sheet flanked by alpha helices on both side | Montreal-kingston bacterial structural genomics initiative | P-loop | Structural genomic
