1twi

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[[Image:1twi.gif|left|200px]]
[[Image:1twi.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1twi |SIZE=350|CAPTION= <scene name='initialview01'>1twi</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1twi", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= LYSA, MJ1097 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0019 LysA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00278 Orn_DAP_Arg_deC]</span>
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{{STRUCTURE_1twi| PDB=1twi | SCENE= }}
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|RELATEDENTRY=[[1tuf|1TUF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twi OCA], [http://www.ebi.ac.uk/pdbsum/1twi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1twi RCSB]</span>
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}}
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'''Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine'''
'''Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine'''
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[[Category: Ray, S S.]]
[[Category: Ray, S S.]]
[[Category: Tomasz, A.]]
[[Category: Tomasz, A.]]
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[[Category: antibiotic resistance]]
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[[Category: Antibiotic resistance]]
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[[Category: diaminopimelate decarboxylase]]
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[[Category: Diaminopimelate decarboxylase]]
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[[Category: lysine biosynthesis]]
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[[Category: Lysine biosynthesis]]
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[[Category: new york structural genomix research consortium]]
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[[Category: New york structural genomix research consortium]]
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[[Category: nysgxrc]]
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[[Category: Nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: t135]]
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[[Category: T135]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:27:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:02:08 2008''
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Revision as of 07:27, 3 May 2008

Image:1twi.gif

Template:STRUCTURE 1twi

Crystal structure of Diaminopimelate Decarboxylase from m. jannaschii in co-complex with L-lysine


Overview

Cocrystal structures of Methanococcus jannaschii diaminopimelate decarboxylase (DAPDC) bound to a substrate analog, azelaic acid, and its L-lysine product have been determined at 2.6 A and 2.0 A, respectively. This PLP-dependent enzyme is responsible for the final step of L-lysine biosynthesis in bacteria and plays a role in beta-lactam antibiotic resistance in Staphylococcus aureus. Substrate specificity derives from recognition of the L-chiral center of diaminopimelate and a system of ionic "molecular rulers" that dictate substrate length. A coupled-enzyme assay system permitted measurement of kinetic parameters for recombinant DAPDCs and inhibition constants (K(i)) for azelaic acid (89 microM) and other substrate analogs. Implications for rational design of broad-spectrum antimicrobial agents targeted against DAPDCs of drug-resistant strains of bacterial pathogens, such as Staphylococcus aureus, are discussed.

About this Structure

1TWI is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor., Ray SS, Bonanno JB, Rajashankar KR, Pinho MG, He G, De Lencastre H, Tomasz A, Burley SK, Structure. 2002 Nov;10(11):1499-508. PMID:12429091 Page seeded by OCA on Sat May 3 10:27:32 2008

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