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Zinc-Dependent Transcriptional Regulator AdcR

Introduction

3TGN space fill model. Blue and white representing symmetric monomers.

AdcR is a zinc-dependent transcriptional regulator that controls the activation of over seventy genes within the bacteria Streptococcus pneumoniae^[1]. Zinc plays a vital role in organism homeostasis acting as a co-factor and a regulator of enzymatic activity, however zinc can lead to cell toxicity and deficiency of other vital metals that are also necessary for protein function^[2]^[3]. Given the many roles zinc plays in general homeostasis the importance of AdcR in Streptococcus pneumoniae can be understood given its ability to regulate zinc transfer proteins within the bacteria.

Proteins 3BPX, 2FBK, 3KP5, and 2PFB (members of the MarR family) are pictured above.

Members of the MarR protein family conserve a number of features including a general triangular shape, a two fold pseudosymmetric homo dimer, and a wingled helix-turn-helix pattern. Consistent with AdcR's identity as a member of the MarR protein family, AdcR exhibits the triangular shape with the wHTH domain (wHTH) binding domain. This structure calls for multiple zinc binding sites that facilitate protein conformational change allowing for DNA binding and regulation through the wHTH domain.

1 Zn(II) Binding
- 1.1 Binding Site 1
- 1.2 Binding Site 2
2 Other Ligands
3 DNA Binding
- 3.1 Hydrogen Bond Network
- 3.2 Helix-Turn-Helix Domain

Zn(II) Binding

Zinc-Dependent Transcriptional Regulator AdcR has on each of its two protomers and can bind a total of four Zn(II) per dimer. is made up of the alpha helix 1, alpha helix 5, and the alpha helix 6. This domain is connected to the HTH winged domain with the long alpha helix 5. This dimerization domain connects to the DNA binding domain and together with the alpha 1 alpha 2 loop make up the ^[4]. Each protomer has one high affinity site (KZn1 = 10^12 M; pH 8) and one low affinity binding site (KZn2 = 10^7 M; pH 8). The metal binding pockets of the AdcR MarR transcriptional regulator are made up of the DNA binding domain with the extended alpha 1 and alpha 2 loop. The two different Zn(II) binding sites are connected via of the Nd1 atom of H108 and then liganding Oe1 atom of E41.

Binding Site 1

consists of a distorted tetrahedral geometry around Zn(II). The four amino acids involved in zinc binding are E24 Oe1, H42 Nd1, H108 Ne2, and H112 Ne2. Binding site 1 is the only binding site that plays a significant role in the protein's regulatory function. The ability of binding site 1 to coordinate to the Zn(II) ion is pH dependent. At pH 6 the binding affinity for the Zn(II) ion is 10^9 - 10^10 M^-1, but at pH 8 the binding affinity increases to 10^12 M^-1. This is due to the charges on the histidines of the binding site. At pH 8, the histidines are positively charged and can interact with the negatively charged Zn(II) ion. However, at pH 6 the histidines are neutrally charged and will not coordinate as well with Zn(II).

Binding Site 2

consists of a highly distorted tetrahedral geometry around the zinc ion. There are three amino acids involved in the binding of the zinc ion (C30, E41, and E107) as well as a water molecule. If a C30A AdcR missense is present in binding site 2, it will have no effect on the ability of the protein to bind DNA. Therefore, binding site 2 has no significant role in DNA binding.

Other Ligands

The AdcR MarR transcriptional regulator is able to bind Co(II) in binding site 1 in a way that induces similar conformational changes to Zn(II) binding. Co(II) coordination in binding site 1 is able to allosterically activate DNA binding similarly to Zn(II) binding.

DNA Binding

Hydrogen Bond Network

The Hydrogen Bonding Network is shown with dotted green lines approximately 2.8 angstroms between residues. The network consists of 4 major residues as follows from right to left: E24, N38, Q40, S74.

The binding of Zinc allows for the conformational change that induces the binding of DNA in order to activate genes. The binding of Zinc metals creates a hydrogen bond network within the protein that connects the metal binding sites and the DNA binding domain. Most importantly, the hydrogen bonding network connects the metal binding pockets to the alpha 4 helix. Alpha 4 helix plays a crucial role in binding DNA because it acts as the recognition helix. The specific sequence of DNA that is recognized by alpha helix 4 is unknown at the moment; however, scientists believe that the hydrogen bond network acts as an allosteric activator for the protein to bind DNA. The hydrogen bond network connects the alpha 2 and alpha 4 helix via hydrogen bonding between specific residues. After zinc is bound, a glutamate (E24) residue from a random coil accepts a hydrogen bond from the carboxamide end of an asparagine (N38) residue from the alpha 2 helix. Then, a glutamine (Q40) residue from alpha 2 helix accepts a hydrogen bond from a serine (S74) residue from the alpha 4 helix. The color coding in the previous sentence represents the , which is seen across the MarR family as a whole.

A charge map of AdcR shows the general triangular shape and the positive charged (blue) area on HTH domains

Helix-Turn-Helix Domain

The AdcR MarR transcriptional regulator's structure resembles the other proteins in the same family as mentioned before; however, the most notable differences are found in the winged helix-turn-helix (wHTH) motif that assists in binding DNA. Although AdcR is a highly alpha helical protein, the "wings" of the DNA binding domain consist of two anti parallel beta strands that are made up of several positively charged residues such as Arg. The major groove of DNA is bound to the recognition helix while the wings grip onto the minor grooves. The charge map on the right highlights the positively charged areas, which stabilize the negatively charged backbone of the major and minor grooves of DNA. There is a wHTH domain on each monomer of the protein.

The domain is made up of the alpha 2 and alpha 4 helices along with anti-parallel beta sheets on each side. Only one monomer is shown. The recognition helix, or the alpha 4 helix, binds the major groove of DNA through hydrogen bonding and Van der Waals interactions between exposed bases. The wings of the helix bind the minor groove of DNA while the other helices stabilize the DNA and Protein upon binding. The two anti parallel beta sheets contain several that stabilize this interaction between DNA.

References

↑ Sanson M, Makthal N, Flores AR, Olsen RJ, Musser JM, Kumaraswami M. Adhesin competence repressor (AdcR) from Streptococcus pyogenes controls adaptive responses to zinc limitation and contributes to virulence. Nucleic Acids Res. 2015 Jan;43(1):418-32. doi: 10.1093/nar/gku1304. Epub 2014 Dec, 15. PMID:25510500 doi:http://dx.doi.org/10.1093/nar/gku1304
↑ Fraústo da Silva J, Williams R. The Biological Chemistry of Elements: The Inorganic Chemistry of Life. Second ed. Oxford University Press; Oxford: 2001.
↑ Ma Z, Jacobsen FE, Giedroc DP. Coordination chemistry of bacterial metal transport and sensing. Chem Rev. 2009 Oct;109(10):4644-81. doi: 10.1021/cr900077w. PMID:19788177 doi:http://dx.doi.org/10.1021/cr900077w
↑ Guerra AJ, Dann CE, Giedroc DP. Crystal Structure of the Zinc-Dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-Bound State. J Am Chem Soc. 2011 Nov 21. PMID:22085181 doi:10.1021/ja2080532

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@@ Line 1: / Line 1: @@
 =='''Zinc-Dependent Transcriptional Regulator AdcR'''==
 ===Introduction===
-AdcR is a zinc-dependent transcriptional regulator that controls the activation of over seventy genes within the bacteria [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae''Streptococcus pneumoniae'']<ref>DOI:10.1093/nar/gku1304 </ref>. Zinc plays a vital role in organism homeostasis acting as a [https://en.wikipedia.org/wiki/Cofactor_(biochemistry) co-factor] and a regulator of enzymatic activity, however zinc can lead to cell toxicity and deficiency of other vital metals that are also necessary for protein function<ref> Fraústo da Silva J, Williams R. The Biological Chemistry of Elements: The Inorganic Chemistry of Life. Second ed. Oxford University Press; Oxford: 2001.</ref><ref> DOI: 10.1021/cr900077w</ref>. Given the importance of zinc in general homeostasis the vital role of AdcR in ''Streptococcus pneumoniae'' can be understood given its ability to regulate zinc transfer proteins within the bacteria.
+[[Image:3TGNspacefill.jpg | 300px | right|thumb|3TGN space fill model. Blue and white representing symmetric monomers.]]
-[[Image:Image1.jpg | 300 px|left|thumb| Members of the MarR protein family conserve a number of features including a general triangular shape, a two fold pseudosymmetric homo dimer, and a wingled helix-turn-helix pattern. Proteins 3BPX, 2FBK, 3KP5, and 2PFB (members of the MarR family) are pictured above.]]
+AdcR is a zinc-dependent transcriptional regulator that controls the activation of over seventy genes within the bacteria [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae''Streptococcus pneumoniae'']<ref>DOI:10.1093/nar/gku1304 </ref>. Zinc plays a vital role in organism homeostasis acting as a [https://en.wikipedia.org/wiki/Cofactor_(biochemistry) co-factor] and a regulator of enzymatic activity, however zinc can lead to cell toxicity and deficiency of other vital metals that are also necessary for protein function<ref> Fraústo da Silva J, Williams R. The Biological Chemistry of Elements: The Inorganic Chemistry of Life. Second ed. Oxford University Press; Oxford: 2001.</ref><ref> DOI: 10.1021/cr900077w</ref>. Given the many roles zinc plays in general homeostasis the importance of AdcR in ''Streptococcus pneumoniae'' can be understood given its ability to regulate zinc transfer proteins within the bacteria.
-Consistent with AdcR's identity as a member of the MarR protein family, AdcR exhibits a triangular shape consisting of a two fold pseudosymetric homo dimer with its own unique winged helix-turn-helix [https://en.wikipedia.org/wiki/Helix-turn-helix (wHTH)] binding domain. This structure calls for multiple zinc binding sites that facilitate protein conformational change allowing for DNA binding and regulation through the wHTH domain.
+[[Image:Image1.jpg | 300 px|left|thumb|Proteins 3BPX, 2FBK, 3KP5, and 2PFB (members of the MarR family) are pictured above.]]
+Members of the MarR protein family conserve a number of features including a general triangular shape, a two fold pseudosymmetric homo dimer, and a wingled helix-turn-helix pattern. Consistent with AdcR's identity as a member of the MarR protein family, AdcR exhibits the triangular shape with the wHTH domain [https://en.wikipedia.org/wiki/Helix-turn-helix (wHTH)] binding domain. This structure calls for multiple zinc binding sites that facilitate protein conformational change allowing for DNA binding and regulation through the wHTH domain.
 <StructureSection load='3TGN' size='350' side='right' caption='3TGN' scene=''>

Sandbox Reserved 1063

From Proteopedia

Revision as of 18:29, 31 March 2017

Zinc-Dependent Transcriptional Regulator AdcR

Introduction

Contents

Zn(II) Binding

Binding Site 1

Binding Site 2

Other Ligands

DNA Binding

Hydrogen Bond Network

Helix-Turn-Helix Domain

References

Views

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