5l21
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of BoNT/A receptor binding domain in complex with VHH C2== | |
| - | + | <StructureSection load='5l21' size='340' side='right' caption='[[5l21]], [[Resolution|resolution]] 1.68Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[5l21]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L21 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr> | |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l21 OCA], [http://pdbe.org/5l21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l21 RCSB], [http://www.ebi.ac.uk/pdbsum/5l21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l21 ProSAT]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH]] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure. | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bontoxilysin]] | ||
| + | [[Category: Jin, R]] | ||
| + | [[Category: Yao, G]] | ||
| + | [[Category: Bont-a-vhh complex]] | ||
| + | [[Category: Botulinum neurotoxin]] | ||
| + | [[Category: Neutralizing antibody]] | ||
| + | [[Category: Toxin]] | ||
| + | [[Category: Vhh]] | ||
Revision as of 10:34, 7 October 2017
Crystal structure of BoNT/A receptor binding domain in complex with VHH C2
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