1u6o

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[[Image:1u6o.gif|left|200px]]
[[Image:1u6o.gif|left|200px]]
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{{Structure
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|PDB= 1u6o |SIZE=350|CAPTION= <scene name='initialview01'>1u6o</scene>
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The line below this paragraph, containing "STRUCTURE_1u6o", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=2BU:(2S,3S)-N6-(2,3,4-TRIHYDROXYBUTYL)-2&#39;-DEOXYADENOSINE+MONO+PHOSPHORIC+ACID'>2BU</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1u6o| PDB=1u6o | SCENE= }}
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|RELATEDENTRY=[[1u6n|1U6N]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6o OCA], [http://www.ebi.ac.uk/pdbsum/1u6o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u6o RCSB]</span>
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'''Mispairing of a Site-Specific Major Groove (2S,3S)-N6-(2,3,4-Trihydroxybutyl)-2-deoxyadenosyl DNA Adduct of Butadiene Diol Epoxide with Deoxyguanosine: Formation of a dA(anti)dG(anti) Pairing Interaction'''
'''Mispairing of a Site-Specific Major Groove (2S,3S)-N6-(2,3,4-Trihydroxybutyl)-2-deoxyadenosyl DNA Adduct of Butadiene Diol Epoxide with Deoxyguanosine: Formation of a dA(anti)dG(anti) Pairing Interaction'''
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==About this Structure==
==About this Structure==
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1U6O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6O OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6O OCA].
==Reference==
==Reference==
Mispairing of a site specific major groove (2S,3S)-N6-(2,3,4-trihydroxybutyl)-2'-deoxyadenosyl DNA Adduct of butadiene diol epoxide with deoxyguanosine: formation of a dA(anti).dG(anti) pairing interaction., Scholdberg TA, Nechev LV, Merritt WK, Harris TM, Harris CM, Lloyd RS, Stone MP, Chem Res Toxicol. 2005 Feb;18(2):145-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15720118 15720118]
Mispairing of a site specific major groove (2S,3S)-N6-(2,3,4-trihydroxybutyl)-2'-deoxyadenosyl DNA Adduct of butadiene diol epoxide with deoxyguanosine: formation of a dA(anti).dG(anti) pairing interaction., Scholdberg TA, Nechev LV, Merritt WK, Harris TM, Harris CM, Lloyd RS, Stone MP, Chem Res Toxicol. 2005 Feb;18(2):145-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15720118 15720118]
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[[Category: Protein complex]]
 
[[Category: Harris, C M.]]
[[Category: Harris, C M.]]
[[Category: Harris, T M.]]
[[Category: Harris, T M.]]
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[[Category: Scholdberg, T A.]]
[[Category: Scholdberg, T A.]]
[[Category: Stone, M P.]]
[[Category: Stone, M P.]]
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[[Category: butadiene]]
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[[Category: Butadiene]]
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[[Category: deoxyadenosyl]]
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[[Category: Deoxyadenosyl]]
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[[Category: diol epoxide]]
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[[Category: Diol epoxide]]
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[[Category: mismatch]]
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[[Category: Mismatch]]
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[[Category: ras61]]
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[[Category: Ras61]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:49:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:06 2008''
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Revision as of 07:49, 3 May 2008

Image:1u6o.gif

Template:STRUCTURE 1u6o

Mispairing of a Site-Specific Major Groove (2S,3S)-N6-(2,3,4-Trihydroxybutyl)-2-deoxyadenosyl DNA Adduct of Butadiene Diol Epoxide with Deoxyguanosine: Formation of a dA(anti)dG(anti) Pairing Interaction


Overview

The (2S,3S)-N6-(2,3,4-trihydroxybutyl)-2'-deoxyadenosyl (BDT) adduct arising from alkylation of adenine N6 by butadiene diol epoxide (BDE) was placed opposite a mismatched deoxyguanosine nucleotide in the complementary strand of the oligodeoxynucleotide 5'-d(CGGACXAGAAG)-3'.5'-d(CTTCTGGTCCG)-3'. This oligodeoxynucleotide contains codon 61 (underlined) of the human N-ras protooncogene. The BDT adduct was at the second position of codon 61, and this was named the ras61 S,S-BDT-(61,2) A.G adduct. NMR spectroscopy revealed the presence of two conformations of the adducted mismatched duplex. In the major conformation, the mismatched base pair X6.G17 was oriented in a "face-to-face" orientation, in which both the modified nucleotide X6 and its complement G17 were intrahelical and in the anti conformation about the glycosyl bond. Hydrogen bonding was suggested between X6 N1 and G17 N1H and between X6 N6H and G17 O6. The presence of the BDT moiety allowed formation of a stable A.G mismatch pair. The identity of the minor conformation could not be determined. If not repaired, the resulting mismatch pair would generate A-->C mutations, which have been associated with this adenine N6 BDT adduct [Carmical, J. R., Nechev, L. N., Harris, C. M., Harris, T. M., and Lloyd, R. S. (2000) Env. Mol. Mutagen. 35, 48-56].

About this Structure

Full crystallographic information is available from OCA.

Reference

Mispairing of a site specific major groove (2S,3S)-N6-(2,3,4-trihydroxybutyl)-2'-deoxyadenosyl DNA Adduct of butadiene diol epoxide with deoxyguanosine: formation of a dA(anti).dG(anti) pairing interaction., Scholdberg TA, Nechev LV, Merritt WK, Harris TM, Harris CM, Lloyd RS, Stone MP, Chem Res Toxicol. 2005 Feb;18(2):145-53. PMID:15720118 Page seeded by OCA on Sat May 3 10:49:30 2008

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