1u7b
From Proteopedia
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'''Crystal structure of hPCNA bound to residues 331-350 of the flap endonuclease-1 (FEN1)''' | '''Crystal structure of hPCNA bound to residues 331-350 of the flap endonuclease-1 (FEN1)''' | ||
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[[Category: Bruning, J B.]] | [[Category: Bruning, J B.]] | ||
[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
| - | [[Category: | + | [[Category: Dna processing]] |
| - | [[Category: | + | [[Category: Fen1]] |
| - | [[Category: | + | [[Category: Flap endonuclease]] |
| - | [[Category: | + | [[Category: Pip-box]] |
| - | [[Category: | + | [[Category: Sliding clamp]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:50:46 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 07:50, 3 May 2008
Crystal structure of hPCNA bound to residues 331-350 of the flap endonuclease-1 (FEN1)
Overview
Human Proliferating Cellular Nuclear Antigen (hPCNA), a member of the sliding clamp family of proteins, makes specific protein-protein interactions with DNA replication and repair proteins through a small peptide motif termed the PCNA-interacting protein, or PIP-box. We solved the structure of hPCNA bound to PIP-box-containing peptides from the p66 subunit of the human replicative DNA polymerase-delta (452-466) at 2.6 A and of the flap endonuclease (FEN1) (331-350) at 1.85 A resolution. Both structures demonstrate that the pol-delta p66 and FEN1 peptides interact with hPCNA at the same site shown to bind the cdk-inhibitor p21(CIP1). Binding studies indicate that peptides from the p66 subunit of the pol-delta holoenzyme and FEN1 bind hPCNA from 189- to 725-fold less tightly than those of p21. Thus, the PIP-box and flanking regions provide a small docking peptide whose affinities can be readily adjusted in accord with biological necessity to mediate the binding of DNA replication and repair proteins to hPCNA.
About this Structure
1U7B is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1., Bruning JB, Shamoo Y, Structure. 2004 Dec;12(12):2209-19. PMID:15576034 Page seeded by OCA on Sat May 3 10:50:46 2008
