1ubi

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{{Structure
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{{STRUCTURE_1ubi| PDB=1ubi | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ubi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ubi OCA], [http://www.ebi.ac.uk/pdbsum/1ubi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ubi RCSB]</span>
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'''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1'''
'''SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1'''
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[[Category: Sawyer, L.]]
[[Category: Sawyer, L.]]
[[Category: Turner, M A.]]
[[Category: Turner, M A.]]
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[[Category: chromosomal protein]]
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[[Category: Chromosomal protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:00:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:58 2008''
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Revision as of 08:00, 3 May 2008

Image:1ubi.jpg

Template:STRUCTURE 1ubi

SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1


Overview

The small protein ubiquitin (76 amino acids) has been synthesized under optimized conditions by Merrifield solid-phase methodology using the N alpha-Fmoc protecting group. The crude polypeptide mixture was purified to homogeneity by gel filtration, dialysis and a combination of cation- and anion-exchange chromatography to yield ubiquitin. Amino acid analysis, enzymic digestion and sequencing by automated Edman degradation were used to authenticate the primary structure. Isoelectric focusing and m.s. were used to demonstrate that the final product was greater than 98% pure with a final yield of 93 mg (4.3%) from a single synthesis on a 0.25 nmol scale.

About this Structure

1UBI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Synthetic, structural and biological studies of the ubiquitin system: the total chemical synthesis of ubiquitin., Ramage R, Green J, Muir TW, Ogunjobi OM, Love S, Shaw K, Biochem J. 1994 Apr 1;299 ( Pt 1):151-8. PMID:8166633 Page seeded by OCA on Sat May 3 11:00:09 2008

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OCA

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