5vf5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the vicilin from Solanum melongena, re-refinement== | |
| + | <StructureSection load='5vf5' size='340' side='right' caption='[[5vf5]], [[Resolution|resolution]] 1.49Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5vf5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_melongena Solanum melongena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VF5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VF5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5cad|5cad]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vf5 OCA], [http://pdbe.org/5vf5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vf5 RCSB], [http://www.ebi.ac.uk/pdbsum/5vf5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vf5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Crystal structure of a vicilin, SM80.1, was determined towards exploring its possible physiological functions. The protein was purified from Solanum melongena by combination of ammonium sulphate fractionation and size exclusion chromatography. Structure was determined ab initio at resolution of 1.5 A by X-ray crystallography showing the three-dimensional topology of the trimeric protein. Each monomer of SM80.1 consists of two similar domains with hydrophobic binding pocket and each accommodating different ligands, i.e. acetate and pyroglutamate. The relatively high stability of these independent anionic ligands in similar pockets indicated a strict requirement of stabilization by hydrogen bonds with the charged residues, suggesting a degree of plasticity within the binding pocket. Comparison of SM80.1 structure with those of other 7S vicilins indicated conservation of putative binding pocket for anionic ligands. Here we propose the possibility of trapping of these ligands in the protein for their requirement in the metabolic processes. | ||
| - | + | Crystal structure of the vicilin from Solanum melongena reveals existence of different anionic ligands in structurally similar pockets.,Jain A, Kumar A, Salunke DM Sci Rep. 2016 Mar 23;6:23600. doi: 10.1038/srep23600. PMID:27004988<ref>PMID:27004988</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5vf5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Solanum melongena]] | ||
| + | [[Category: Dauter, Z]] | ||
| + | [[Category: Jaskolski, M]] | ||
| + | [[Category: Minor, W]] | ||
| + | [[Category: Porebski, P J]] | ||
| + | [[Category: Pozharski, E]] | ||
| + | [[Category: Rupp, B]] | ||
| + | [[Category: Stanfield, R]] | ||
| + | [[Category: Weichenberger, C X]] | ||
| + | [[Category: Wlodawer, A]] | ||
| + | [[Category: 7s vicilin sm80 1 plant protein sequence assignment re-refinement]] | ||
| + | [[Category: Plant protein]] | ||
Revision as of 07:15, 6 December 2017
Crystal structure of the vicilin from Solanum melongena, re-refinement
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