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5wyg

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Revision as of 07:20, 7 October 2020

The crystal structure of the apo form of Mtb MazF

Structural highlights

5wyg is a 2 chain structure with sequence from Myctu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	mazF7, Rv2063A (MYCTU)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MAZF7_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an endoribonuclease (By similarity).[UniProtKB:P9WIH9]^[1] ^[2]

Publication Abstract from PubMed

Tuberculosis (TB) is a severe disease caused by Mycobacterium tuberculosis (M. tb) and the well-characterized M. tb MazE/F proteins play important roles in stress adaptation. Recently, the MazF-mt9 toxin has been found to display endonuclease activities towards tRNAs but the mechanism is unknown. We hereby present the crystal structure of apo-MazF-mt9. The enzyme recognizes tRNALys with a central UUU motif within the anticodon loop, but is insensitive to the sequence context outside of the loop. Based on our crystallographic and biochemical studies, we identified key residues for catalysis and proposed the potential tRNA-binding site.

Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis.,Chen R, Tu J, Liu Z, Meng F, Ma P, Ding Z, Yang C, Chen L, Deng X, Xie W Biochem Biophys Res Commun. 2017 May 6;486(3):804-810. doi:, 10.1016/j.bbrc.2017.03.132. Epub 2017 Mar 25. PMID:28351618^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gupta A. Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol Lett. 2009 Jan;290(1):45-53. doi:, 10.1111/j.1574-6968.2008.01400.x. Epub 2008 Nov 10. PMID:19016878 doi:http://dx.doi.org/10.1111/j.1574-6968.2008.01400.x
↑ Ramage HR, Connolly LE, Cox JS. Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009 Dec;5(12):e1000767. doi: 10.1371/journal.pgen.1000767. Epub 2009, Dec 11. PMID:20011113 doi:http://dx.doi.org/10.1371/journal.pgen.1000767
↑ Chen R, Tu J, Liu Z, Meng F, Ma P, Ding Z, Yang C, Chen L, Deng X, Xie W. Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis. Biochem Biophys Res Commun. 2017 May 6;486(3):804-810. doi:, 10.1016/j.bbrc.2017.03.132. Epub 2017 Mar 25. PMID:28351618 doi:http://dx.doi.org/10.1016/j.bbrc.2017.03.132

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wyg"

@@ Line 1: / Line 1: @@
 ==The crystal structure of the apo form of Mtb MazF==
-<StructureSection load='5wyg' size='340' side='right' caption='[[5wyg]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
+<StructureSection load='5wyg' size='340' side='right'caption='[[5wyg]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wyg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WYG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WYG FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wyg OCA], [http://pdbe.org/5wyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wyg RCSB], [http://www.ebi.ac.uk/pdbsum/5wyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wyg ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mazF7, Rv2063A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wyg OCA], [http://pdbe.org/5wyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wyg RCSB], [http://www.ebi.ac.uk/pdbsum/5wyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wyg ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Myctu]]
 [[Category: Chen, R]]
 [[Category: Tu, J]]

5wyg

From Proteopedia

Revision as of 07:20, 7 October 2020

The crystal structure of the apo form of Mtb MazF

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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