1ue4
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ue4.gif|left|200px]] | [[Image:1ue4.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ue4", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1ue4| PDB=1ue4 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of d(GCGAAAGC)''' | '''Crystal structure of d(GCGAAAGC)''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE4 OCA]. | |
==Reference== | ==Reference== | ||
Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G.A pairs to form a functional G.AxA.G crossing., Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):422-31. Epub 2004, Feb 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14993665 14993665] | Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G.A pairs to form a functional G.AxA.G crossing., Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):422-31. Epub 2004, Feb 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14993665 14993665] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Hirao, I.]] | [[Category: Hirao, I.]] | ||
[[Category: Kondo, J.]] | [[Category: Kondo, J.]] | ||
| Line 30: | Line 26: | ||
[[Category: Takenaka, A.]] | [[Category: Takenaka, A.]] | ||
[[Category: Watanabe, K.]] | [[Category: Watanabe, K.]] | ||
| - | [[Category: | + | [[Category: Bulge-in duplex]] |
| - | [[Category: | + | [[Category: Dna]] |
| - | [[Category: | + | [[Category: G:axa:g crossing]] |
| - | [[Category: | + | [[Category: Sheared g:a pair]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:05:41 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:05, 3 May 2008
Crystal structure of d(GCGAAAGC)
Overview
The DNA fragments d(GCGAAGC) and d(GCGAAAGC) are known to exhibit several extraordinary properties. Their crystal structures have been determined at 1.6 and 1.65 A resolution, respectively. Two heptamers aligned in an antiparallel fashion associate to form a duplex having molecular twofold symmetry. In the crystallographic asymmetric unit, there are three structurally identical duplexes. At both ends of each duplex, two Watson-Crick G.C pairs constitute the stem regions. In the central part, two sheared G.A pairs are crossed and stacked on each other, so that the stacked two guanine bases of the G.AxA.G crossing expose their Watson-Crick and major-groove sites into solvent, suggesting a functional role. The adenine moieties of the A(5) residues are inside the duplex, wedged between the A(4) and G(6) residues, but there are no partners for interactions. To close the open space on the counter strand, the duplex is strongly bent. In the asymmetric unit of the d(GCGAAAGC) crystal (tetragonal form), there is only one octamer chain. However, the two chains related by the crystallographic twofold symmetry associate to form an antiparallel duplex, similar to the base-intercalated duplex found in the hexagonal crystal form of the octamer. It is interesting to note that the significant difference between the present bulge-in structure of d(GCGAAGC) and the base-intercalated duplex of d(GCGAAAGC) can be ascribed to a switching of partners of the sheared G.A pairs.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G.A pairs to form a functional G.AxA.G crossing., Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):422-31. Epub 2004, Feb 25. PMID:14993665 Page seeded by OCA on Sat May 3 11:05:41 2008
