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1umh

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[[Image:1umh.gif|left|200px]]
[[Image:1umh.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1umh |SIZE=350|CAPTION= <scene name='initialview01'>1umh</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1umh", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= mouse ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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|DOMAIN=
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{{STRUCTURE_1umh| PDB=1umh | SCENE= }}
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|RELATEDENTRY=[[1umi|1UMI]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1umh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umh OCA], [http://www.ebi.ac.uk/pdbsum/1umh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1umh RCSB]</span>
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'''Structural basis of sugar-recognizing ubiquitin ligase'''
'''Structural basis of sugar-recognizing ubiquitin ligase'''
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[[Category: Yamaguchi, Y.]]
[[Category: Yamaguchi, Y.]]
[[Category: Yoshida, Y.]]
[[Category: Yoshida, Y.]]
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[[Category: lectin]]
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[[Category: Lectin]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: scf]]
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[[Category: Scf]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: ubiquitin]]
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[[Category: Ubiquitin]]
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[[Category: ubiquitin ligase]]
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[[Category: Ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:25:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:12:18 2008''
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Revision as of 08:25, 3 May 2008

Image:1umh.gif

Template:STRUCTURE 1umh

Structural basis of sugar-recognizing ubiquitin ligase


Overview

SCF(Fbs1) is a ubiquitin ligase that functions in the endoplasmic reticulum (ER)-associated degradation pathway. Fbs1/Fbx2, a member of the F-box proteins, recognizes high-mannose oligosaccharides. Efficient binding to an N-glycan requires di-N-acetylchitobiose (chitobiose). Here we report the crystal structures of the sugar-binding domain (SBD) of Fbs1 alone and in complex with chitobiose. The SBD is composed of a ten-stranded antiparallel beta-sandwich. The structure of the SBD-chitobiose complex includes hydrogen bonds between Fbs1 and chitobiose and insertion of the methyl group of chitobiose into a small hydrophobic pocket of Fbs1. Moreover, NMR spectroscopy has demonstrated that the amino acid residues adjoining the chitobiose-binding site interact with the outer branches of the carbohydrate moiety. Considering that the innermost chitobiose moieties in N-glycans are usually involved in intramolecular interactions with the polypeptide moieties, we propose that Fbs1 interacts with the chitobiose in unfolded N-glycoprotein, pointing the protein moiety toward E2 for ubiquitination.

About this Structure

1UMH is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis of sugar-recognizing ubiquitin ligase., Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K, Nat Struct Mol Biol. 2004 Apr;11(4):365-70. Epub 2004 Feb 29. PMID:14990996 Page seeded by OCA on Sat May 3 11:25:37 2008

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