1uo9

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[[Image:1uo9.jpg|left|200px]]
[[Image:1uo9.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1uo9 |SIZE=350|CAPTION= <scene name='initialview01'>1uo9</scene>, resolution 1.5&Aring;
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The line below this paragraph, containing "STRUCTURE_1uo9", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Sin+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deacetoxycephalosporin-C_synthase Deacetoxycephalosporin-C synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.20.1 1.14.20.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1uo9| PDB=1uo9 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uo9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uo9 OCA], [http://www.ebi.ac.uk/pdbsum/1uo9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uo9 RCSB]</span>
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}}
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'''DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE'''
'''DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE'''
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[[Category: Scheltinga, A C.Terwisscha Van.]]
[[Category: Scheltinga, A C.Terwisscha Van.]]
[[Category: Valegard, K.]]
[[Category: Valegard, K.]]
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[[Category: antibiotic biosynthesis]]
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[[Category: Antibiotic biosynthesis]]
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[[Category: iron]]
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[[Category: Iron]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: vitamin c]]
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[[Category: Vitamin c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:29:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:13:02 2008''
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Revision as of 08:29, 3 May 2008

Image:1uo9.jpg

Template:STRUCTURE 1uo9

DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE


Overview

Deacetoxycephalosporin-C synthase (DAOCS) is a mononuclear ferrous enzyme that transforms penicillins into cephalosporins by inserting a carbon atom into the penicillin nucleus. In the first half-reaction, dioxygen and 2-oxoglutarate produce a reactive iron-oxygen species, succinate and CO2. The oxidizing iron species subsequently reacts with penicillin to give cephalosporin and water. Here we describe high-resolution structures for ferrous DAOCS in complex with penicillins, the cephalosporin product, the cosubstrate and the coproduct. Steady-state kinetic data, quantum-chemical calculations and the new structures indicate a reaction sequence in which a 'booby-trapped' oxidizing species is formed. This species is stabilized by the negative charge of succinate on the iron. The binding sites of succinate and penicillin overlap, and when penicillin replaces succinate, it removes the stabilizing charge, eliciting oxidative attack on itself. Requisite groups of penicillin are within 1 A of the expected position of a ferryl oxygen in the enzyme-penicillin complex.

About this Structure

1UO9 is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.

Reference

The structural basis of cephalosporin formation in a mononuclear ferrous enzyme., Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I, Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:14718929 Page seeded by OCA on Sat May 3 11:29:33 2008

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