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1uon
From Proteopedia
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[[Image:1uon.gif|left|200px]] | [[Image:1uon.gif|left|200px]] | ||
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'''REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION''' | '''REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION''' | ||
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[[Category: Walker, S B.]] | [[Category: Walker, S B.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
| - | [[Category: | + | [[Category: Core protein]] |
| - | [[Category: | + | [[Category: Cryoem]] |
| - | [[Category: | + | [[Category: Polymerase]] |
| - | [[Category: | + | [[Category: Reovirus]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:30:19 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:30, 3 May 2008
REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION
Overview
Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral polymerases packaged within the viral core to transcribe its ten distinct plus-strand RNAs. To localize these polymerases, the structure of the reovirion was refined to a resolution of 7.6 A by cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction. X-ray crystal models of reovirus proteins, including polymerase lambda 3, were then fitted into the density map. Each copy of lambda 3 was found anchored to the inner surface of the icosahedral core shell, making major contacts with three molecules of shell protein lambda 1 and overlapping, but not centering on, a five-fold axis. The overlap explains why only one copy of lambda 3 is bound per vertex. lambda 3 is furthermore oriented with its transcript exit channel facing a small channel through the lambda 1 shell, suggesting how the nascent RNA is passed into the large external cavity of the pentameric capping enzyme complex formed by protein lambda 2.
About this Structure
1UON is a Protein complex structure of sequences from Reovirus sp.. Full crystallographic information is available from OCA.
Reference
Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 A., Zhang X, Walker SB, Chipman PR, Nibert ML, Baker TS, Nat Struct Biol. 2003 Dec;10(12):1011-8. Epub 2003 Nov 9. PMID:14608373 Page seeded by OCA on Sat May 3 11:30:19 2008
