1uow
From Proteopedia
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'''CALCIUM BINDING DOMAIN C2B''' | '''CALCIUM BINDING DOMAIN C2B''' | ||
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[[Category: Sequeira, S M.]] | [[Category: Sequeira, S M.]] | ||
[[Category: Sollner, T H.]] | [[Category: Sollner, T H.]] | ||
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| - | [[Category: | + | [[Category: Transmembrane]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:30:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:30, 3 May 2008
CALCIUM BINDING DOMAIN C2B
Overview
Synaptotagmin I has two tandem Ca(2+)-binding C(2) domains, which are essential for fast synchronous synaptic transmission in the central nervous system. We have solved four crystal structures of the C(2)B domain, one of them in the cation-free form at 1.50 A resolution, two in the Ca(2+)-bound form at 1.04 A (two bound Ca(2+) ions) and 1.65 A (three bound Ca(2+) ions) resolution and one in the Sr(2+)-bound form at 1.18 A (one bound Sr(2+) ion) resolution. The side chains of four highly conserved aspartic acids (D303, D309, D363, and D365) and two main chain oxygens (M302:O and Y364:O), together with water molecules, are in direct contact with two bound Ca(2+) ions (sites 1 and 2). At higher Ca(2+) concentrations, the side chain of N333 rotates and cooperates with D309 to generate a third Ca(2+) coordination site (site 3). Divalent cation binding sites 1 and 2 in the C(2)B domain were previously identified from NMR NOE patterns and titration studies, supplemented by site-directed mutation analysis. One difference between the crystal and NMR studies involves D371, which is not involved in coordination with any of the identified Ca(2+) sites in the crystal structures, while it is coordinated to Ca(2+) in site 2 in the NMR structure. In the presence of Sr(2+), which is also capable of triggering exocytosis, but with lower efficiency, only one cation binding site (site 1) was occupied in the crystallographic structure.
About this Structure
1UOW is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystallographic identification of Ca2+ and Sr2+ coordination sites in synaptotagmin I C2B domain., Cheng Y, Sequeira SM, Malinina L, Tereshko V, Sollner TH, Patel DJ, Protein Sci. 2004 Oct;13(10):2665-72. Epub 2004 Aug 31. PMID:15340165 Page seeded by OCA on Sat May 3 11:30:57 2008
