1urx

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[[Image:1urx.gif|left|200px]]
[[Image:1urx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1urx |SIZE=350|CAPTION= <scene name='initialview01'>1urx</scene>, resolution 1.7&Aring;
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The line below this paragraph, containing "STRUCTURE_1urx", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AAL:3,6-ANHYDRO-L-GALACTOSE'>AAL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1urx| PDB=1urx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1urx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1urx OCA], [http://www.ebi.ac.uk/pdbsum/1urx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1urx RCSB]</span>
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}}
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'''CRYSTALLOGRAPHIC STRUCTURE OF BETA-AGARASE A IN COMPLEX WITH OLIGOAGAROSE'''
'''CRYSTALLOGRAPHIC STRUCTURE OF BETA-AGARASE A IN COMPLEX WITH OLIGOAGAROSE'''
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[[Category: Helbert, W.]]
[[Category: Helbert, W.]]
[[Category: Henrissat, B.]]
[[Category: Henrissat, B.]]
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[[Category: agarose]]
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[[Category: Agarose]]
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[[Category: beta-agarase]]
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[[Category: Beta-agarase]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: family 16]]
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[[Category: Family 16]]
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[[Category: glycoside hydrolase]]
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[[Category: Glycoside hydrolase]]
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[[Category: two binding-site]]
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[[Category: Two binding-site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:36:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:14:20 2008''
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Revision as of 08:36, 3 May 2008

Image:1urx.gif

Template:STRUCTURE 1urx

CRYSTALLOGRAPHIC STRUCTURE OF BETA-AGARASE A IN COMPLEX WITH OLIGOAGAROSE


Overview

Agarose is a gel-forming polysaccharide with an alpha-L(1,4)-3,6-anhydro-galactose, beta-D(1,3)-galactose repeat unit, from the cell walls of marine red algae. beta-agarase A, from the Gram-negative bacterium Zobellia galactanivorans, is secreted to the external medium and degrades agarose with an endo-mechanism. The structure of the inactive mutant beta-agarase A-E147S in complex with agaro-octaose has been solved at 1.7 A resolution. Two oligosaccharide chains are bound to the protein. The first one resides in the active site channel, spanning subsites -4 to -1. A second oligosaccharide binding site, on the opposite side of the protein, was filled with eight sugar units, parallel to the active site. The crystal structure of the beta-agarase A with agaro-octaose provides detailed information on agarose recognition in the catalytic site. The presence of the second, parallel, binding site suggests that the enzyme might be able to unwind the double-helical structure of agarose prior to the catalytic cleavage.

About this Structure

1URX is a Single protein structure of sequence from Zobellia galactanivorans. Full crystallographic information is available from OCA.

Reference

Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose., Allouch J, Helbert W, Henrissat B, Czjzek M, Structure. 2004 Apr;12(4):623-32. PMID:15062085 Page seeded by OCA on Sat May 3 11:36:22 2008

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