1uum

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[[Image:1uum.jpg|left|200px]]
[[Image:1uum.jpg|left|200px]]
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{{Structure
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|PDB= 1uum |SIZE=350|CAPTION= <scene name='initialview01'>1uum</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1uum", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Bog+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AFI:2-[4-(4-CHLOROPHENYL)CYCLOHEXYLIDENE]-3,4-DIHYDROXY-1(2H)-NAPHTHALENONE'>AFI</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=ORO:OROTIC+ACID'>ORO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydroorotate_dehydrogenase Dihydroorotate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.11 1.3.99.11] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1uum| PDB=1uum | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uum OCA], [http://www.ebi.ac.uk/pdbsum/1uum PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uum RCSB]</span>
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'''RAT DIHYDROOROTATE DEHYDROGENASE (DHOD)IN COMPLEX WITH ATOVAQUONE'''
'''RAT DIHYDROOROTATE DEHYDROGENASE (DHOD)IN COMPLEX WITH ATOVAQUONE'''
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[[Category: Nours, J Le.]]
[[Category: Nours, J Le.]]
[[Category: Ullrich, A.]]
[[Category: Ullrich, A.]]
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[[Category: atovaquone]]
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[[Category: Atovaquone]]
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[[Category: brequinar]]
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[[Category: Brequinar]]
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[[Category: dihydroorotate dehydrogenase]]
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[[Category: Dihydroorotate dehydrogenase]]
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[[Category: fad]]
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[[Category: Fad]]
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[[Category: flavoprotein]]
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[[Category: Flavoprotein]]
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[[Category: nucleotide metabolism]]
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[[Category: Nucleotide metabolism]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: pyrimidine biosynthesis]]
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[[Category: Pyrimidine biosynthesis]]
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[[Category: transit peptide]]
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[[Category: Transit peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:42:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:15:30 2008''
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Revision as of 08:42, 3 May 2008

Image:1uum.jpg

Template:STRUCTURE 1uum

RAT DIHYDROOROTATE DEHYDROGENASE (DHOD)IN COMPLEX WITH ATOVAQUONE


Overview

The flavin enzyme dihydroorotate dehydrogenase (DHOD; EC 1.3.99.11) catalyzes the oxidation of dihydroorotate to orotate, the fourth step in the de novo pyrimidine biosynthesis of UMP. The enzyme is a promising target for drug design in different biological and clinical applications for cancer and arthritis. The first crystal structure of the class 2 dihydroorotate dehydrogenase from rat has been determined in complex with its two inhibitors brequinar and atovaquone. These inhibitors have shown promising results as anti-proliferative, immunosuppressive, and antiparasitic agents. A unique feature of the class 2 DHODs is their N-terminal extension, which folds into a separate domain comprising two alpha-helices. This domain serves as the binding site for the two inhibitors and the respiratory quinones acting as the second substrate for the class 2 DHODs. The orientation of the first N-terminal helix is very different in the two complexes of rat DHOD (DHODR). Binding of atovaquone causes a 12 A movement of the first residue in the first alpha-helix. Based on the information from the two structures of DHODR, a model for binding of the quinone and the residues important for the interactions could be defined. His 56 and Arg 136, which are fully conserved in all class 2 DHODs, seem to play a key role in the interaction with the electron acceptor. The differences between the membrane-bound rat DHOD and membrane-associated class 2 DHODs exemplified by the Escherichia coli DHOD has been investigated by GRID computations of the hydrophobic probes predicted to interact with the membrane.

About this Structure

1UUM is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.

Reference

Inhibitor binding in a class 2 dihydroorotate dehydrogenase causes variations in the membrane-associated N-terminal domain., Hansen M, Le Nours J, Johansson E, Antal T, Ullrich A, Loffler M, Larsen S, Protein Sci. 2004 Apr;13(4):1031-42. PMID:15044733 Page seeded by OCA on Sat May 3 11:42:55 2008

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