This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3zj8

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 16:36, 27 February 2019

Crystal structure of strictosidine glucosidase in complex with inhibitor-2

Structural highlights

3zj8 is a 2 chain structure with sequence from Ophioxylon serpentinum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3zj6, 3zj7
Activity:	3-alpha-(S)-strictosidine beta-glucosidase, with EC number 3.2.1.105
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SG1_RAUSE] Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the therapy of heart disorders.^[1]

Publication Abstract from PubMed

Abstract Insight into the structure and inhibition mechanism of O-beta-d-glucosidases by deoxa-pyranosylamine type inhibitors is provided by X-ray analysis of complexes between raucaffricine and strictosidine glucosidases and N-(cyclohexylmethyl)-, N-(cyclohexyl)- and N-(bromobenzyl)-beta-d-gluco-1,5-deoxa-pyranosylamine. All inhibitors anchored exclusively in the catalytic active site by competition with appropriate enzyme substrates. Thus facilitated prospective elucidation of the binding networks with residues located at <3.9 A distance will enable the development of potent inhibitors suitable for the production of valuable alkaloid glucosides, raucaffricine and strictosidine, by means of synthesis in Rauvolfia serpentina cell suspension cultures.

Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours.,Xia L, Lin H, Staniek A, Panjikar S, Ruppert M, Hilgers P, Williardt J, Rajendran C, Wang M, Warzecha H, Jager V, Stockigt J J Enzyme Inhib Med Chem. 2014 Aug 20:1-7. PMID:25140865^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stockigt J. Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity. ACS Chem Biol. 2011 Oct 28. PMID:22004291 doi:10.1021/cb200267w
↑ Xia L, Lin H, Staniek A, Panjikar S, Ruppert M, Hilgers P, Williardt J, Rajendran C, Wang M, Warzecha H, Jager V, Stockigt J. Ligand structures of synthetic deoxa-pyranosylamines with raucaffricine and strictosidine glucosidases provide structural insights into their binding and inhibitory behaviours. J Enzyme Inhib Med Chem. 2014 Aug 20:1-7. PMID:25140865 doi:http://dx.doi.org/10.3109/14756366.2014.949252

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zj8"

@@ Line 3: / Line 3: @@
 <StructureSection load='3zj8' size='340' side='right' caption='[[3zj8]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Devilpepper Devilpepper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZJ8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zj8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZJ8 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LR1:(1R,2S,3S,4R,5R)-4-[(4-BROMOPHENYL)METHYLAMINO]-5-(HYDROXYMETHYL)CYCLOPENTANE-1,2,3-TRIOL'>LR1</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zj6|3zj6]], [[3zj7|3zj7]]</td></tr>
@@ Line 27: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Devilpepper]]
+[[Category: Ophioxylon serpentinum]]
 [[Category: Castiglia, A]]
 [[Category: Jaeger, V]]

3zj8

From Proteopedia

Revision as of 16:36, 27 February 2019

Crystal structure of strictosidine glucosidase in complex with inhibitor-2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox