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5n8o
From Proteopedia
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8o OCA], [http://pdbe.org/5n8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8o RCSB], [http://www.ebi.ac.uk/pdbsum/5n8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8o ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8o OCA], [http://pdbe.org/5n8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8o RCSB], [http://www.ebi.ac.uk/pdbsum/5n8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8o ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Relaxases play essential roles in conjugation, the main process by which bacteria exchange genetic material, notably antibiotic resistance genes. They are bifunctional enzymes containing a trans-esterase activity, which is responsible for nicking the DNA strand to be transferred and for covalent attachment to the resulting 5'-phosphate end, and a helicase activity, which is responsible for unwinding the DNA while it is being transported to a recipient cell. Here we show that these two activities are carried out by two conformers that can both load simultaneously on the origin of transfer DNA. We solve the structure of one of these conformers by cryo electron microscopy to near-atomic resolution, elucidating the molecular basis of helicase function by relaxases and revealing insights into the mechanistic events taking place in the cell prior to substrate transport during conjugation. | ||
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| + | Cryo-EM Structure of a Relaxase Reveals the Molecular Basis of DNA Unwinding during Bacterial Conjugation.,Ilangovan A, Kay CWM, Roier S, El Mkami H, Salvadori E, Zechner EL, Zanetti G, Waksman G Cell. 2017 May 4;169(4):708-721.e12. doi: 10.1016/j.cell.2017.04.010. Epub 2017, Apr 27. PMID:28457609<ref>PMID:28457609</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5n8o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:21, 10 May 2017
Cryo EM structure of the conjugative relaxes TraI of the F/R1 plasmid system
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Categories: Ilangovan, A | Waksman, G | Zanetti, G | Cryo em | Helicase | Relaxase | Transferase | Translocase
