1v2z
From Proteopedia
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'''Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA''' | '''Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA''' | ||
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[[Category: Shibata, H.]] | [[Category: Shibata, H.]] | ||
[[Category: Uzumaki, T.]] | [[Category: Uzumaki, T.]] | ||
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| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rsgi]] |
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Revision as of 09:00, 3 May 2008
Crystal structure of the C-terminal domain of Thermosynechococcus elongatus BP-1 KaiA
Overview
KaiA, KaiB and KaiC constitute the circadian clock machinery in cyanobacteria, and KaiA activates kaiBC expression whereas KaiC represses it. Here we show that KaiA is composed of three functional domains, the N-terminal amplitude-amplifier domain, the central period-adjuster domain and the C-terminal clock-oscillator domain. The C-terminal domain is responsible for dimer formation, binding to KaiC, enhancing KaiC phosphorylation and generating the circadian oscillations. The X-ray crystal structure at a resolution of 1.8 A of the C-terminal clock-oscillator domain of KaiA from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 shows that residue His270, located at the center of a KaiA dimer concavity, is essential to KaiA function. KaiA binding to KaiC probably occurs via the concave surface. On the basis of the structure, we predict the structural roles of the residues that affect circadian oscillations.
About this Structure
1V2Z is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.
Reference
Crystal structure of the C-terminal clock-oscillator domain of the cyanobacterial KaiA protein., Uzumaki T, Fujita M, Nakatsu T, Hayashi F, Shibata H, Itoh N, Kato H, Ishiura M, Nat Struct Mol Biol. 2004 Jul;11(7):623-31. Epub 2004 May 30. PMID:15170179 Page seeded by OCA on Sat May 3 12:00:41 2008
