5kh8
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structures of the apo state fluoride riboswitch== | |
| + | <StructureSection load='5kh8' size='340' side='right' caption='[[5kh8]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5kh8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KH8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kh8 OCA], [http://pdbe.org/5kh8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kh8 RCSB], [http://www.ebi.ac.uk/pdbsum/5kh8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kh8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Riboswitches control gene expression through ligand-dependent structural rearrangements of the sensing aptamer domain. However, we found that the Bacillus cereus fluoride riboswitch aptamer adopts identical tertiary structures in solution with and without ligand. Using chemical-exchange saturation transfer (CEST) NMR spectroscopy, we revealed that the structured ligand-free aptamer transiently accesses a low-populated ( approximately 1%) and short-lived ( approximately 3 ms) excited conformational state that unravels a conserved 'linchpin' base pair to signal transcription termination. Upon fluoride binding, this highly localized, fleeting process is allosterically suppressed, which activates transcription. We demonstrated that this mechanism confers effective fluoride-dependent gene activation over a wide range of transcription rates, which is essential for robust toxicity responses across diverse cellular conditions. These results unveil a novel switching mechanism that employs ligand-dependent suppression of an aptamer excited state to coordinate regulatory conformational transitions rather than adopting distinct aptamer ground-state tertiary architectures, exemplifying a new mode of ligand-dependent RNA regulation. | ||
| - | + | An excited state underlies gene regulation of a transcriptional riboswitch.,Zhao B, Guffy SL, Williams B, Zhang Q Nat Chem Biol. 2017 Jul 17. doi: 10.1038/nchembio.2427. PMID:28719589<ref>PMID:28719589</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5kh8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Zhang, Q]] | ||
| + | [[Category: Zhao, B]] | ||
| + | [[Category: Apo state]] | ||
| + | [[Category: Pseudoknot]] | ||
| + | [[Category: Riboswitch aptamer]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Transcriptional regulation]] | ||
Revision as of 03:51, 4 August 2017
Solution structures of the apo state fluoride riboswitch
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