1vjq
From Proteopedia
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[[Image:1vjq.gif|left|200px]] | [[Image:1vjq.gif|left|200px]] | ||
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'''Designed protein based on backbone conformation of procarboxypeptidase-A (1AYE) with sidechains chosen for maximal predicted stability.''' | '''Designed protein based on backbone conformation of procarboxypeptidase-A (1AYE) with sidechains chosen for maximal predicted stability.''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VJQ OCA]. | |
==Reference== | ==Reference== | ||
A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins., Dantas G, Kuhlman B, Callender D, Wong M, Baker D, J Mol Biol. 2003 Sep 12;332(2):449-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12948494 12948494] | A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins., Dantas G, Kuhlman B, Callender D, Wong M, Baker D, J Mol Biol. 2003 Sep 12;332(2):449-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12948494 12948494] | ||
| - | [[Category: ]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Baker, D.]] | [[Category: Baker, D.]] | ||
[[Category: Merritt, E A.]] | [[Category: Merritt, E A.]] | ||
[[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]] | [[Category: SGPP, Structural Genomics of Pathogenic Protozoa Consortium.]] | ||
| - | [[Category: | + | [[Category: Engineered protein]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Sgpp]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Structural genomics of pathogenic protozoa consortium]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:36:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 09:36, 3 May 2008
Designed protein based on backbone conformation of procarboxypeptidase-A (1AYE) with sidechains chosen for maximal predicted stability.
Overview
A previously developed computer program for protein design, RosettaDesign, was used to predict low free energy sequences for nine naturally occurring protein backbones. RosettaDesign had no knowledge of the naturally occurring sequences and on average 65% of the residues in the designed sequences differ from wild-type. Synthetic genes for ten completely redesigned proteins were generated, and the proteins were expressed, purified, and then characterized using circular dichroism, chemical and temperature denaturation and NMR experiments. Although high-resolution structures have not yet been determined, eight of these proteins appear to be folded and their circular dichroism spectra are similar to those of their wild-type counterparts. Six of the proteins have stabilities equal to or up to 7kcal/mol greater than their wild-type counterparts, and four of the proteins have NMR spectra consistent with a well-packed, rigid structure. These encouraging results indicate that the computational protein design methods can, with significant reliability, identify amino acid sequences compatible with a target protein backbone.
About this Structure
Full crystallographic information is available from OCA.
Reference
A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins., Dantas G, Kuhlman B, Callender D, Wong M, Baker D, J Mol Biol. 2003 Sep 12;332(2):449-60. PMID:12948494 Page seeded by OCA on Sat May 3 12:36:36 2008
