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4uxe

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== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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The phage-proximal part of the long tail fibres of bacteriophage T4 consists of a trimer of the 1289 amino-acid gene product 34 (gp34). Different carboxy-terminal parts of gp34 have been produced and crystallized. Crystals of gp34(726-1289) diffracting X-rays to 2.9 A resolution, crystals of gp34(781-1289) diffracting to 1.9 A resolution and crystals of gp34(894-1289) diffracting to 3.0 and 2.0 A resolution and belonging to different crystal forms were obtained. Native data were collected for gp34(726-1289) and gp34(894-1289), while single-wavelength anomalous diffraction data were collected for selenomethionine-containing gp34(781-1289) and gp34(894-1289). For the latter, high-quality anomalous signal was obtained.
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Long tail fibers of bacteriophage T4 are formed by proteins gp34, gp35, gp36, and gp37, with gp34 located at the phage-proximal end and gp37 at the phage-distal, receptor-binding end. We have solved the structure of the carboxy-terminal region of gp34, consisting of amino acids 894-1289, by single-wavelength anomalous diffraction and extended the structure to amino acids 744-1289 using data collected from crystals containing longer gp34-fragments. The structure reveals three repeats of a mixed alpha-beta fibrous domain in residues 744 to 877. A triple-helical neck connects to an extended triple beta-helix domain (amino acids 900-1127) punctuated by two beta-prism domains. Next, a beta-prism domain decorated with short helices and extended beta-helices is present (residues 1146-1238), while the C-terminal end is capped with another short beta-helical region and three beta-hairpins. The structure provides insight into the stability of the fibrous gp34 protein.
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Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34.,Granell M, Namura M, Alvira S, Garcia-Doval C, Singh AK, Gutsche I, van Raaij MJ, Kanamaru S Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):970-5. doi:, 10.1107/S2053230X14010449. Epub 2014 Jun 19. PMID:25005101<ref>PMID:25005101</ref>
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Crystal Structure of the Carboxy-Terminal Region of the Bacteriophage T4 Proximal Long Tail Fiber Protein Gp34.,Granell M, Namura M, Alvira S, Kanamaru S, van Raaij MJ Viruses. 2017 Jun 30;9(7). pii: E168. doi: 10.3390/v9070168. PMID:28665339<ref>PMID:28665339</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

Revision as of 16:35, 20 October 2017

Crystal structure of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34, P21 selenomethionine crystal

4uxe, resolution 2.00Å

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