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GP1 of Lassa Virus

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'''GP1''' (Glycoprotein 1) is the receptor binding domain of Lassa virus that mediates receptor recognition. Research thus far indicates that GP1 from Lassa virus may undergo irreversible conformational changes that could serve as an immunological decoy mechanism.
'''GP1''' (Glycoprotein 1) is the receptor binding domain of Lassa virus that mediates receptor recognition. Research thus far indicates that GP1 from Lassa virus may undergo irreversible conformational changes that could serve as an immunological decoy mechanism.
==Structural Highlights==
==Structural Highlights==
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Protein structure accession number: '''4ZJF'''. 4ZJF is a 4 chain structure with <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> ligands attached to each chain. The overall architecture of GP1 features a central β-sheet and two distinct halves: a glycosylated half containing the receptor-binding site that is made mostly by the central β-sheet and surrounding loops and a half that contains mostly helices and most likely faces the trimer axis<ref name="MolMechforLAMP1">DOI 10.1128/JVI.00651-15</ref>..
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Protein structure accession number: '''4ZJF'''. 4ZJF is a 4 chain structure with <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> ligands attached to each chain. The overall architecture of GP1 features a central β-sheet and two distinct halves: a glycosylated half containing the receptor-binding site that is made mostly by the central β-sheet and surrounding loops and a half that contains mostly helices and most likely faces the trimer axis<ref name="MolMechforLAMP1">DOI 10.1128/JVI.00651-15</ref>. The method to determine this structure was
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[https://en.wikipedia.org/wiki/X-ray_crystallography X-ray diffraction]
=== Histidine Triad===
=== Histidine Triad===
Attached to this structure is a <scene name='76/761695/Histriad/4'>unique triad of histidines</scene> that is highly conserved among Old World arenaviruses. Located on the β-sheet face of GP1, the histidine triad is a structural element that directly interacts with [[LAMP1]] and helps stabilize a LAMP1-"compatible" conformation by providing a molecular mechanism for the pH-dependent receptor switching<ref name="MolMechforLAMP1" />.
Attached to this structure is a <scene name='76/761695/Histriad/4'>unique triad of histidines</scene> that is highly conserved among Old World arenaviruses. Located on the β-sheet face of GP1, the histidine triad is a structural element that directly interacts with [[LAMP1]] and helps stabilize a LAMP1-"compatible" conformation by providing a molecular mechanism for the pH-dependent receptor switching<ref name="MolMechforLAMP1" />.

Revision as of 08:58, 26 June 2017

4ZJF structure

Drag the structure with the mouse to rotate

References

  1. ↑ 1.0 1.1 Cohen-Dvashi H, Cohen N, Israeli H, Diskin R. Molecular mechanism for LAMP1 recognition by Lassa Virus. J Virol. 2015 May 13. pii: JVI.00651-15. PMID:25972533 doi:http://dx.doi.org/10.1128/JVI.00651-15

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Rebecca Holstein, Michal Harel

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