Luciferase

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<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
== Function ==
== Function ==
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[[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>. See detailed analysis in<br />
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[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>. See detailed analysis in<br />
* [[Luciola cruciata luciferase]]<br />
* [[Luciola cruciata luciferase]]<br />
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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{{#tree:id=OrganizedByTopic|openlevels=0|
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[[3iep]], [[1lci]], [[5dv9]], [[3ier]] – fLuc – firefly<br />
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* Luciferase
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[[5kyt]], [[5kyv]] – fLuc (mutant)<br />
+
-
[[5dwv]] – fLuc+ substrate analog<br />
+
-
[[1ba3]] – fLuc+bromoform<br />
+
-
[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br />
+
-
[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br />
+
-
[[4g37]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br />
+
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[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br />
+
-
[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br />
+
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[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br />
+
-
[[3qya]] – LtLuc (mutant) – ''Lampyris turkestanicus''<br />
+
-
[[4m46]] – LtLuc<br />
+
-
[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br />
+
-
[[1luc]], [[1brl]] - VhLuc α+β chain<br />
+
-
[[1bsl]], [[1xkj]] - VhLuc β chain<br />
+
-
[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
+
 +
**[[1ba3]] – fLuc+bromoform - firefly<br />
 +
**[[3qya]] – LtLuc (mutant) – ''Lampyris turkestanicus''<br />
 +
**[[4m46]] – LtLuc<br />
 +
**[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br />
 +
**[[1luc]], [[1brl]] - VhLuc α+β chain<br />
 +
**[[1bsl]], [[1xkj]] - VhLuc β chain<br />
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 +
*Luciferin 2-monooxygenase
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 +
**[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
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**[[5b0u]] – Luc2mo (mutant) catalytic subunit – luminous shrimp<br />
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 +
*Luciferin 4-monooxygenase
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 +
**[[3iep]], [[1lci]], [[5dv9]], [[3ier]] – fLuc <br />
 +
**[[5kyt]], [[5kyv]] – fLuc (mutant)<br />
 +
**[[5dwv]] – fLuc+ substrate analog<br />
 +
**[[3ies]], [[3rix]], [[4e5d]] – fLuc+inhibitor<br />
 +
**[[4g36]] – fLUc + dehydroluciferyl-sulfamoyl adenosine<br />
 +
**[[4g37]] – fLUc (mutant) + dehydroluciferyl-sulfamoyl adenosine<br />
 +
**[[5gz2]] – fLuc + thiazole derivative<br />
 +
**[[5gyz]] – fLuc + AMP + thiazole derivative<br />
 +
**[[5wys]] – fLuc + inhibitor<br />
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**[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br />
 +
**[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br />
 +
**[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br />
 +
**[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
 +
}}

Revision as of 08:44, 31 July 2018

Luciferase (PDB code 3iep)

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References

  1. ↑ Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123-42. PMID:2030669
  2. ↑ Gould SJ, Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal Biochem. 1988 Nov 15;175(1):5-13. PMID:3072883
  3. ↑ Auld DS, Lovell S, Thorne N, Lea WA, Maloney DJ, Shen M, Rai G, Battaile KP, Thomas CJ, Simeonov A, Hanzlik RP, Inglese J. Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124. Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):4878-83. Epub 2010 Mar 1. PMID:20194791
  4. ↑ Campbell ZT, Weichsel A, Montfort WR, Baldwin TO. Crystal Structure of the Bacterial Luciferase:Flavin Complex Provides Insight into the Function of the subunit. Biochemistry. 2009 May 12. PMID:19435287 doi:http://dx.doi.org/10.1021/bi900003t


Additional Literature

  • Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
  • Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8

External Resources

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