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Transcription-repair coupling factor
From Proteopedia
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== Function == | == Function == | ||
| - | '''Transcription-repair coupling factor (TRCF)''' enables the coupling of these processes in bacteria and humans. The TRCF has ATPase activity. <scene name='2eyq/Asymmunit/2'>The crystallographic asymmetric unit of the solved structure contains 2 monomers of Mfd (residues 2-1147 of 1151 for one molecule and 5-1147 of the other), five HEPES molecules, and the sulfate ions.</scene> | + | '''Transcription-repair coupling factor (TRCF)''' or '''Mfd''' enables the coupling of these processes in bacteria and humans. The TRCF has ATPase activity. <scene name='2eyq/Asymmunit/2'>The crystallographic asymmetric unit of the solved structure contains 2 monomers of Mfd (residues 2-1147 of 1151 for one molecule and 5-1147 of the other), five HEPES molecules, and the sulfate ions.</scene> |
Size exclusion chromatogaphy indicates that the protein <scene name='2eyq/Biolunit/1'>exists as a monomer in solution.</scene> | Size exclusion chromatogaphy indicates that the protein <scene name='2eyq/Biolunit/1'>exists as a monomer in solution.</scene> | ||
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[[2eyq]], [[2b2n]] – EcTRCF – ''Escherichia coli''<br /> | [[2eyq]], [[2b2n]] – EcTRCF – ''Escherichia coli''<br /> | ||
[[3hjh]] – EcTRCF residues 1-470<br /> | [[3hjh]] – EcTRCF residues 1-470<br /> | ||
| - | [[4dfc]] – EcTRCF D2 domain + UvrABC system protein A<br /> | + | [[6yhz]] - EcTRCF residues 472-547 – NMR<br /> |
| - | [[3mlq]] – | + | [[4dfc]] – EcTRCF D2 domain 127-213 + UvrABC system protein A<br /> |
| + | [[6xeo]] – EcTRCF + DNA – Cryo EM<br /> | ||
| + | [[3mlq]] – TtTRCF RNA polymerase interacting domain + DNA-directed RNA polymerase subunit β - ''Thermus thermophilus''<br /> | ||
| + | [[6m6a]] – TtTRCF + RNA polymerase – Cryo EM<br /> | ||
| + | [[6m6b]] – TtTRCF + RNA polymerase + ATP-γ-S – Cryo EM<br /> | ||
[[2qsr]] – TRCF C terminal – ''Streptococcus pneumoni''<br /> | [[2qsr]] – TRCF C terminal – ''Streptococcus pneumoni''<br /> | ||
| + | [[6ac6]], [[6aca]], [[6ac8]] – MsTRCF – ''Mycobacterium smegmatis''<br /> | ||
| + | [[6acx]] – MsTRCF + ADP<br /> | ||
| + | |||
</StructureSection> | </StructureSection> | ||
Revision as of 09:58, 8 December 2020
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Reference
- Deaconescu AM, Chambers AL, Smith AJ, Nickels BE, Hochschild A, Savery NJ, Darst SA. Structural basis for bacterial transcription-coupled DNA repair. Cell. 2006 Feb 10;124(3):507-20. PMID:16469698 doi:10.1016/j.cell.2005.11.045
Created with the participation of Wayne Decatur
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Karsten Theis, Michal Harel, Alexander Berchansky, Wayne Decatur
