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5ejj

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Revision as of 07:01, 22 April 2020

Crystal structure of UfSP from C.elegans

Structural highlights

5ejj is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UFSP_CAEEL] Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ufm-1, a ubiquitin-like modifier protein bound to a number of target proteins (By similarity). Required, with oct-4, for the localization of a subset of 7 transmembrane domain odorant receptors, including odr-10, to the cilia of olfactory neurons AWA and AWC. Operates in aggregation behavior, and responses to oxygen levels.[UniProtKB:Q9NUQ7]^[1] ^[2] ^[3]

Publication Abstract from PubMed

Ubiquitin-fold modifier 1 (Ufm1) specific protease (UfSP) is a novel cysteine protease that activates Ufm1 from its precursor by processing the C-terminus to expose the conserved Gly necessary for substrate conjugation and de-conjugates Ufm1 from the substrate. There are two forms: UfSP1 and UfSP2, the later with an additional domain at the N-terminus. Ufm1 and both the conjugating and deconjugating enzymes are highly conserved. However, in Caenorhabditis elegans there is one UfSP which has extra 136 residues at the N terminus compared to UfSP2. The crystal structure of cUfSP reveals that these additional residues display a MPN fold while the rest of the structure mimics that of UfSP2. The MPN domain does not have the metalloprotease activity found in some MPN-domain containing protein, rather it is required for the recognition and deufmylation of the substrate of cUfSP, UfBP1. In addition, the MPN domain is also required for localization to the endoplasmic reticulum.

The MPN domain of Caenorhabditis elegans UfSP modulates both substrate recognition and deufmylation activity.,Ha BH, Kim KH, Yoo HM, Lee W, EunKyeong Kim E Biochem Biophys Res Commun. 2016 Aug 5;476(4):450-6. doi:, 10.1016/j.bbrc.2016.05.143. Epub 2016 May 28. PMID:27240952^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ de Bono M, Tobin DM, Davis MW, Avery L, Bargmann CI. Social feeding in Caenorhabditis elegans is induced by neurons that detect aversive stimuli. Nature. 2002 Oct 31;419(6910):899-903. PMID:12410303 doi:http://dx.doi.org/10.1038/nature01169
↑ Chen C, Itakura E, Weber KP, Hegde RS, de Bono M. An ER complex of ODR-4 and ODR-8/Ufm1 specific protease 2 promotes GPCR maturation by a Ufm1-independent mechanism. PLoS Genet. 2014 Mar 6;10(3):e1004082. doi: 10.1371/journal.pgen.1004082., eCollection 2014 Mar. PMID:24603482 doi:http://dx.doi.org/10.1371/journal.pgen.1004082
↑ Dwyer ND, Troemel ER, Sengupta P, Bargmann CI. Odorant receptor localization to olfactory cilia is mediated by ODR-4, a novel membrane-associated protein. Cell. 1998 May 1;93(3):455-66. PMID:9590179
↑ Ha BH, Kim KH, Yoo HM, Lee W, EunKyeong Kim E. The MPN domain of Caenorhabditis elegans UfSP modulates both substrate recognition and deufmylation activity. Biochem Biophys Res Commun. 2016 Aug 5;476(4):450-6. doi:, 10.1016/j.bbrc.2016.05.143. Epub 2016 May 28. PMID:27240952 doi:http://dx.doi.org/10.1016/j.bbrc.2016.05.143

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ejj"

@@ Line 1: / Line 1: @@
 ==Crystal structure of UfSP from C.elegans==
-<StructureSection load='5ejj' size='340' side='right' caption='[[5ejj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='5ejj' size='340' side='right'caption='[[5ejj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ejj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EJJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EJJ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [http://pdbe.org/5ejj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [http://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ejj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ejj OCA], [http://pdbe.org/5ejj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ejj RCSB], [http://www.ebi.ac.uk/pdbsum/5ejj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ejj ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Ha, B]]
 [[Category: Kim, E E]]

5ejj

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Revision as of 07:01, 22 April 2020

Crystal structure of UfSP from C.elegans

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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