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1w3m

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[[Image:1w3m.gif|left|200px]]
[[Image:1w3m.gif|left|200px]]
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{{Structure
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|PDB= 1w3m |SIZE=350|CAPTION= <scene name='initialview01'>1w3m</scene>, resolution 1.00&Aring;
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|LIGAND= <scene name='pdbligand=3MD:2S,3S-3-METHYLASPARTIC+ACID'>3MD</scene>, <scene name='pdbligand=ACB:3-METHYL-ASPARTIC+ACID'>ACB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPI:6-CARBOXYPIPERIDINE'>CPI</scene>, <scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=LNG:12-METHYLTRIDECANOIC+ACID'>LNG</scene>, <scene name='pdbligand=VDL:(2R,3R)-2,3-DIAMINOBUTANOIC+ACID'>VDL</scene>, <scene name='pdbligand=VLL:(2S)-2,3-DIAMINOBUTANOIC+ACID'>VLL</scene>
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{{STRUCTURE_1w3m| PDB=1w3m | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w3m OCA], [http://www.ebi.ac.uk/pdbsum/1w3m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w3m RCSB]</span>
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'''CRYSTAL STRUCTURE OF TSUSHIMYCIN'''
'''CRYSTAL STRUCTURE OF TSUSHIMYCIN'''
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[[Category: Sheldrick, G M.]]
[[Category: Sheldrick, G M.]]
[[Category: Vertesy, L.]]
[[Category: Vertesy, L.]]
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[[Category: amphomycin]]
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[[Category: Amphomycin]]
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[[Category: antibiotic]]
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[[Category: Antibiotic]]
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[[Category: daptomycin]]
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[[Category: Daptomycin]]
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[[Category: lipopetide]]
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[[Category: Lipopetide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:06:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:30:46 2008''
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Revision as of 10:06, 3 May 2008

Template:STRUCTURE 1w3m

CRYSTAL STRUCTURE OF TSUSHIMYCIN


Overview

The amphomycin derivative tsushimycin has been crystallized and its structure determined at 1.0 A resolution. The asymmetric unit contains 12 molecules and with 1300 independent atoms this structure is one of the largest solved using ab initio direct methods. The antibiotic is comprised of a cyclodecapeptide core, an exocyclic amino acid and a fatty-acid residue. Its backbone adopts a saddle-like conformation that is stabilized by a Ca2+ ion bound within the peptide ring and accounts for the Ca2+-dependence of this antibiotic class. Additional Ca2+ ions link the antibiotic molecules to dimers that enclose an empty space resembling a binding cleft. The dimers possess a large hydrophobic surface capable of interacting with the bacterial cell membrane. The antibiotic daptomycin may exhibit a similar conformation, as the amino-acid sequence is conserved at positions involved in Ca2+ binding.

About this Structure

1W3M is a Single protein structure of sequence from Actinoplanes friuliensis. Full crystallographic information is available from OCA.

Reference

Structure of the lipopeptide antibiotic tsushimycin., Bunkoczi G, Vertesy L, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1160-4. Epub 2005, Jul 20. PMID:16041082 Page seeded by OCA on Sat May 3 13:06:44 2008

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