1w7k
From Proteopedia
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'''E.COLI FOLC IN COMPLEX WITH ADP, WITHOUT FOLATE SUBSTRATE''' | '''E.COLI FOLC IN COMPLEX WITH ADP, WITHOUT FOLATE SUBSTRATE''' | ||
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[[Category: Vincent, S.]] | [[Category: Vincent, S.]] | ||
[[Category: Viviani, F.]] | [[Category: Viviani, F.]] | ||
| - | [[Category: | + | [[Category: Atp-binding]] |
| - | [[Category: | + | [[Category: Dhf]] |
| - | [[Category: | + | [[Category: Dihydrofolate synthase]] |
| - | [[Category: | + | [[Category: Folate biosynthesis]] |
| - | [[Category: | + | [[Category: Folc]] |
| - | [[Category: | + | [[Category: Ligase]] |
| - | [[Category: | + | [[Category: Multifunctional enzyme]] |
| - | [[Category: | + | [[Category: Synthase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:15:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:15, 3 May 2008
E.COLI FOLC IN COMPLEX WITH ADP, WITHOUT FOLATE SUBSTRATE
Overview
In some bacteria, such as Escherichia coli, the addition of L-glutamate to dihydropteroate (dihydrofolate synthetase activity) and the subsequent additions of L-glutamate to tetrahydrofolate (folylpolyglutamate synthetase (FPGS) activity) are catalyzed by the same enzyme, FolC. The crystal structure of E. coli FolC is described in this paper. It showed strong similarities to that of the FPGS enzyme of Lactobacillus casei within the ATP binding site and the catalytic site, as do all other members of the Mur synthethase superfamily. FolC structure revealed an unexpected dihydropteroate binding site very different from the folate site identified previously in the FPGS structure. The relevance of this site is exemplified by the presence of phosphorylated dihydropteroate, a reaction intermediate in the DHFS reaction. L. casei FPGS is considered a relevant model for human FPGS. As such, the presence of a folate binding site in E. coli FolC, which is different from the one seen in FPGS enzymes, provides avenues for the design of specific inhibitors of this enzyme in antimicrobial therapy.
About this Structure
1W7K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Escherichia coli FolC structure reveals an unexpected dihydrofolate binding site providing an attractive target for anti-microbial therapy., Mathieu M, Debousker G, Vincent S, Viviani F, Bamas-Jacques N, Mikol V, J Biol Chem. 2005 May 13;280(19):18916-22. Epub 2005 Feb 10. PMID:15705579 Page seeded by OCA on Sat May 3 13:15:57 2008
