1w7z
From Proteopedia
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'''CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)''' | '''CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)''' | ||
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[[Category: Schneider, T R.]] | [[Category: Schneider, T R.]] | ||
[[Category: Uson, I.]] | [[Category: Uson, I.]] | ||
| - | [[Category: | + | [[Category: Cystein knot]] |
| - | [[Category: | + | [[Category: Ecballium elaterium]] |
| - | [[Category: | + | [[Category: Protease inhibitor]] |
| - | [[Category: | + | [[Category: Squash seed inhibitor]] |
| - | [[Category: | + | [[Category: Trypsin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:16:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:16, 3 May 2008
CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)
Overview
The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.
About this Structure
1W7Z is a Single protein structure of sequence from Ecballium elaterium. Full crystallographic information is available from OCA.
Reference
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold., Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759 Page seeded by OCA on Sat May 3 13:16:54 2008
