1w98

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1w98.gif|left|200px]]
[[Image:1w98.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1w98 |SIZE=350|CAPTION= <scene name='initialview01'>1w98</scene>, resolution 2.15&Aring;
+
The line below this paragraph, containing "STRUCTURE_1w98", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1w98| PDB=1w98 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w98 OCA], [http://www.ebi.ac.uk/pdbsum/1w98 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w98 RCSB]</span>
+
-
}}
+
'''THE STRUCTURAL BASIS OF CDK2 ACTIVATION BY CYCLIN E'''
'''THE STRUCTURAL BASIS OF CDK2 ACTIVATION BY CYCLIN E'''
Line 32: Line 29:
[[Category: Lowe, E D.]]
[[Category: Lowe, E D.]]
[[Category: Skamnaki, V.]]
[[Category: Skamnaki, V.]]
-
[[Category: cell cycle]]
+
[[Category: Cell cycle]]
-
[[Category: cyclin dependant kinase]]
+
[[Category: Cyclin dependant kinase]]
-
[[Category: cyclin]]
+
[[Category: Cyclin]]
-
[[Category: protein kinase]]
+
[[Category: Protein kinase]]
-
[[Category: transferase]]
+
[[Category: Transferase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:19:56 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:32:58 2008''
+

Revision as of 10:19, 3 May 2008

Image:1w98.gif

Template:STRUCTURE 1w98

THE STRUCTURAL BASIS OF CDK2 ACTIVATION BY CYCLIN E


Overview

Cyclin E, an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression in metazoans and is frequently overexpressed in cancer cells. It is essential for entry to the cell cycle from G0 quiescent phase, for the assembly of prereplication complexes and for endoreduplication in megakaryotes and giant trophoblast cells. We report the crystal structure of pCDK2 in complex with a truncated cyclin E1 (residues 81-363) at 2.25 A resolution. The N-terminal cyclin box fold of cyclin E1 is similar to that of cyclin A and promotes identical changes in pCDK2 that lead to kinase activation. The C-terminal cyclin box fold shows significant differences from cyclin A. It makes additional interactions with pCDK2, especially in the region of the activation segment, and contributes to CDK2-independent binding sites of cyclin E. Kinetic analysis with model peptide substrates show a 1.6-fold increase in kcat for pCDK2/cyclin E1 (81-363) over kcat of pCDK2/cyclin E (full length) and pCDK2/cyclin A. The structural and kinetic results indicate no inherent substrate discrimination between pCDK2/cyclin E and pCDK2/cyclin A with model substrates.

About this Structure

1W98 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles., Honda R, Lowe ED, Dubinina E, Skamnaki V, Cook A, Brown NR, Johnson LN, EMBO J. 2005 Feb 9;24(3):452-63. Epub 2005 Jan 20. PMID:15660127 Page seeded by OCA on Sat May 3 13:19:56 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w98"
Personal tools