1w9c
From Proteopedia
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'''PROTEOLYTIC FRAGMENT OF CRM1 SPANNING SIX C-TERMINAL HEAT REPEATS''' | '''PROTEOLYTIC FRAGMENT OF CRM1 SPANNING SIX C-TERMINAL HEAT REPEATS''' | ||
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[[Category: Soler-Lopez, M.]] | [[Category: Soler-Lopez, M.]] | ||
[[Category: Steuerwald, U.]] | [[Category: Steuerwald, U.]] | ||
| - | [[Category: | + | [[Category: Exportin 1]] |
| - | [[Category: | + | [[Category: Nuclear export complex]] |
| - | [[Category: | + | [[Category: Nuclear protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:20:14 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:20, 3 May 2008
PROTEOLYTIC FRAGMENT OF CRM1 SPANNING SIX C-TERMINAL HEAT REPEATS
Overview
CRM1/Exportin1 mediates the nuclear export of proteins bearing a leucine-rich nuclear export signal (NES) by forming a cooperative ternary complex with the NES-bearing substrate and the small GTPase Ran. We present a structural model of human CRM1 based on a combination of X-ray crystallography, homology modeling, and electron microscopy. The architecture of CRM1 resembles that of the import receptor transportin1, with 19 HEAT repeats and a large loop implicated in Ran binding. Residues critical for NES recognition are identified adjacent to the cysteine residue targeted by leptomycin B (LMB), a specific CRM1 inhibitor. We present evidence that a conformational change of the Ran binding loop accounts for the cooperativity of Ran- and substrate binding and for the selective enhancement of CRM1-mediated export by the cofactor RanBP3. Our findings indicate that a single architectural and mechanistic framework can explain the divergent effects of RanGTP on substrate binding by many import and export receptors.
About this Structure
1W9C is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex., Petosa C, Schoehn G, Askjaer P, Bauer U, Moulin M, Steuerwald U, Soler-Lopez M, Baudin F, Mattaj IW, Muller CW, Mol Cell. 2004 Dec 3;16(5):761-75. PMID:15574331 Page seeded by OCA on Sat May 3 13:20:14 2008
