1wb5
From Proteopedia
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[[Image:1wb5.gif|left|200px]] | [[Image:1wb5.gif|left|200px]] | ||
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| - | | | + | {{STRUCTURE_1wb5| PDB=1wb5 | SCENE= }} |
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'''S954A MUTANT OF THE FERULOYL ESTERASE MODULE FROM CLOSTRIDIUM THERMOCELLUM COMPLEXED WITH SYRINGATE''' | '''S954A MUTANT OF THE FERULOYL ESTERASE MODULE FROM CLOSTRIDIUM THERMOCELLUM COMPLEXED WITH SYRINGATE''' | ||
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[[Category: Prates, J A.]] | [[Category: Prates, J A.]] | ||
[[Category: Tarbouriech, N.]] | [[Category: Tarbouriech, N.]] | ||
| - | [[Category: | + | [[Category: Esterase family 1]] |
| - | [[Category: | + | [[Category: Ferulic acid]] |
| - | [[Category: | + | [[Category: Glycosidase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | [[Category: | + | [[Category: Repeat]] |
| - | [[Category: | + | [[Category: Xylan degradation]] |
| - | [[Category: | + | [[Category: Xylanase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:24:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:24, 3 May 2008
S954A MUTANT OF THE FERULOYL ESTERASE MODULE FROM CLOSTRIDIUM THERMOCELLUM COMPLEXED WITH SYRINGATE
Overview
Feruloyl esterases play a key role in the degradation of the intricate structure of the plant cell wall by hydrolysing the ferulate ester groups involved in the cross-linking between hemicelluloses and between hemicellulose and lignin. The structure of the feruloyl esterase module of Clostridium thermocellum cellulosomal xylanase 10B has been reported previously. It displays the alpha/beta hydrolase fold with a classical Ser-His-Asp catalytic triad. Here, the structures of a Ser-Ala mutant of this feruloyl esterase in complexes with methyl syringate, methyl sinapinate and methyl vanillate are described. Substrate binding is accompanied by subtle conformational changes at amino acids Trp982, Met955, Asn1023 and Ile1019 in the ligand-binding cavity. The structural determinants, particularly the m-methoxy substituent, governing the substrate specificity of Xyn10B feruloyl esterase are rationalized.
About this Structure
1WB5 is a Single protein structure of sequence from Clostridium thermocellum. Full crystallographic information is available from OCA.
Reference
Molecular determinants of substrate specificity in the feruloyl esterase module of xylanase 10B from Clostridium thermocellum., Tarbouriech N, Prates JA, Fontes CM, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2005 Feb;61(Pt 2):194-7. Epub 2005, Jan 19. PMID:15681871 Page seeded by OCA on Sat May 3 13:24:37 2008
