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1wl7
From Proteopedia
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[[Image:1wl7.gif|left|200px]] | [[Image:1wl7.gif|left|200px]] | ||
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'''Structure of the thermostable arabinanase''' | '''Structure of the thermostable arabinanase''' | ||
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[[Category: Tada, T.]] | [[Category: Tada, T.]] | ||
[[Category: Yamaguchi, A.]] | [[Category: Yamaguchi, A.]] | ||
| - | [[Category: | + | [[Category: Abn-t]] |
| - | [[Category: | + | [[Category: Arabinanase]] |
| - | [[Category: | + | [[Category: Bacillus]] |
| - | [[Category: | + | [[Category: Glycoside hydrolase]] |
| - | [[Category: | + | [[Category: Thermostable enzyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:49:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:49, 3 May 2008
Structure of the thermostable arabinanase
Overview
The crystal structure of a thermostable endo-1,5-alpha-L-arabinanase, ABN-TS, from Bacillus thermodenitrificans TS-3 was determined at 1.9 A to an R-factor of 18.3% and an R-free-factor of 22.5%. The enzyme molecule has a five-bladed beta-propeller fold. The substrate-binding cleft formed across one face of the propeller is open on both sides to allow random binding of several sugar units in the polymeric substrate arabinan. The beta-propeller fold is stabilized through a ring closure. ABN-TS exhibits a new closure-mode involving residues in the N-terminal region: Phe7 to Gly21 exhibit hydrogen bonds and hydrophobic interactions with the first and last blades, and Phe4 links the second and third blades through a hydrogen bond and an aromatic stacking interaction, respectively. The role of the N-terminal region in the thermostability was confirmed with a mutant lacking 16 amino acid residues from the N-terminus of ABN-TS.
About this Structure
1WL7 is a Single protein structure of sequence from Geobacillus thermodenitrificans. Full crystallographic information is available from OCA.
Reference
Structural basis for thermostability of endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3., Yamaguchi A, Tada T, Wada K, Nakaniwa T, Kitatani T, Sogabe Y, Takao M, Sakai T, Nishimura K, J Biochem. 2005 May;137(5):587-92. PMID:15944411 Page seeded by OCA on Sat May 3 13:49:55 2008
