1wnu

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[[Image:1wnu.gif|left|200px]]
[[Image:1wnu.gif|left|200px]]
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{{Structure
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|PDB= 1wnu |SIZE=350|CAPTION= <scene name='initialview01'>1wnu</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1wnu", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|GENE= PH0574 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])
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|DOMAIN=
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{{STRUCTURE_1wnu| PDB=1wnu | SCENE= }}
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|RELATEDENTRY=[[1v7o|1V7O]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnu OCA], [http://www.ebi.ac.uk/pdbsum/1wnu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wnu RCSB]</span>
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'''Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine'''
'''Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine'''
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[[Category: Tanaka, I.]]
[[Category: Tanaka, I.]]
[[Category: Yao, M.]]
[[Category: Yao, M.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:55:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:38:45 2008''
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Revision as of 10:55, 3 May 2008

Image:1wnu.gif

Template:STRUCTURE 1wnu

Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine


Overview

AlaX is the homologue of the class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA(Ala). Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc. Together with mutational analysis, we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine is responsible for the discrimination of noncognate serine from cognate alanine, based on their chemical natures. Furthermore, we confirmed that the conserved Gln-584 in alanyl-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala).

About this Structure

1WNU is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Molecular basis of alanine discrimination in editing site., Sokabe M, Okada A, Yao M, Nakashima T, Tanaka I, Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11669-74. Epub 2005 Aug 8. PMID:16087889 Page seeded by OCA on Sat May 3 13:55:35 2008

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