1wo2

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[[Image:1wo2.gif|left|200px]]
[[Image:1wo2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1wo2 |SIZE=350|CAPTION= <scene name='initialview01'>1wo2</scene>, resolution 2.01&Aring;
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The line below this paragraph, containing "STRUCTURE_1wo2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1wo2| PDB=1wo2 | SCENE= }}
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|RELATEDENTRY=[[1hx0|1HX0]], [[1ua3|1UA3]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wo2 OCA], [http://www.ebi.ac.uk/pdbsum/1wo2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wo2 RCSB]</span>
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}}
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'''Crystal structure of the pig pancreatic alpha-amylase complexed with malto-oligosaacharides under the effect of the chloride ion'''
'''Crystal structure of the pig pancreatic alpha-amylase complexed with malto-oligosaacharides under the effect of the chloride ion'''
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[[Category: Payan, F.]]
[[Category: Payan, F.]]
[[Category: Qian, M.]]
[[Category: Qian, M.]]
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[[Category: beta-alpha-barrel]]
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[[Category: Beta-alpha-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:55:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:38:51 2008''
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Revision as of 10:56, 3 May 2008

Image:1wo2.gif

Template:STRUCTURE 1wo2

Crystal structure of the pig pancreatic alpha-amylase complexed with malto-oligosaacharides under the effect of the chloride ion


Overview

Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with maltopentaose using a chloride-free buffer was analyzed by X-ray crystallography. A conspicuous reorientation of the acid/base catalyst Glu233 residue was found to occur. The structural results, along with kinetic data, show that the acid/base catalyst is maintained in the active site, in an optimum position, pointing toward the scissile bond-atom, due to the presence of chloride ions. The present study therefore explains the mechanism of PPA activation by chloride ions.

About this Structure

1WO2 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase., Qian M, Ajandouz el H, Payan F, Nahoum V, Biochemistry. 2005 Mar 8;44(9):3194-201. PMID:15736930 Page seeded by OCA on Sat May 3 13:55:59 2008

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