1hkh
From Proteopedia
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| - | [[Image:1hkh. | + | [[Image:1hkh.jpg|left|200px]]<br /><applet load="1hkh" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1hkh" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1hkh, resolution 1.73Å" /> | caption="1hkh, resolution 1.73Å" /> | ||
'''UNLIGATED GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES'''<br /> | '''UNLIGATED GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HKH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Microbacterium Microbacterium] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1HKH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Microbacterium Microbacterium] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CA:So4 Binding Site For Chain B'>CA</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: co-factor free haloperoxidase]] | [[Category: co-factor free haloperoxidase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:29:35 2007'' |
Revision as of 14:19, 18 December 2007
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UNLIGATED GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES
Overview
The structure of the recombinant (-) gamma-lactamase from an, Aureobacterium species has been solved at 1.73A resolution in the cubic, space group F23 with unit cell parameters a=b=c=240.6A. The trimeric, enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor, free haloperoxidases. The structure has been solved in complex with a, covalently bound ligand originating from the host cell and also in the, unligated form. The associated density in the former structure has been, interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo, [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the, catalytic Ser98. Soaks of these crystals with the industrial substrate, gamma-lactam or its structural analogue, norcamphor, result in the, displacement of the ligand from the enzyme active site, thereby allowing, determination of the unligated structure. The presence of the ligand in, the active site protects the enzyme from serine hydrolase inhibitors., Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a, substrate of the enzyme.
About this Structure
1HKH is a Single protein structure of sequence from Microbacterium with SO4 as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site., Line K, Isupov MN, Littlechild JA, J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810
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