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5mx5

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Revision as of 07:31, 19 August 2020

Mouse PA28alpha-beta

Structural highlights

5mx5 is a 14 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	5msj, 5msk
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PSME1_MOUSE] Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. [PSME2_MOUSE] Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Publication Abstract from PubMed

The heptameric proteasome activator (PA) 28alphabeta is known to modulate class I antigen processing by docking onto 20S proteasome core particles (CPs). The exact stoichiometry and arrangement of its alpha and beta subunits, however, is still controversial. Here we analyzed murine PA28 complexes regarding structure and assembly. Strikingly, PA28alpha, PA28beta, and PA28alphabeta preparations form heptamers, but solely PA28alpha and PA28alphabeta associate with CPs. Co-expression of alpha and beta yields one unique PA28alphabeta species with an unchangeable subunit composition. Structural data on PA28alpha, PA28beta, and PA28alphabeta up to 2.9 A resolution reveal a PA28alpha4beta3 complex with an alternating subunit arrangement and a single alpha-alpha interface. Differential scanning fluorimetry experiments and activity assays classify PA28alpha4beta3 as most stable and most active, indicating that this assembly might represent the physiologically relevant species. Together, our data resolve subunit composition and arrangement of PA28alphabeta and clarify how an asymmetric heptamer can be assembled from two highly homologous subunits.

The Mammalian Proteasome Activator PA28 Forms an Asymmetric alpha4beta3 Complex.,Huber EM, Groll M Structure. 2017 Aug 22. pii: S0969-2126(17)30247-2. doi:, 10.1016/j.str.2017.07.013. PMID:28867616^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huber EM, Groll M. The Mammalian Proteasome Activator PA28 Forms an Asymmetric alpha4beta3 Complex. Structure. 2017 Aug 22. pii: S0969-2126(17)30247-2. doi:, 10.1016/j.str.2017.07.013. PMID:28867616 doi:http://dx.doi.org/10.1016/j.str.2017.07.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5mx5"

@@ Line 1: / Line 1: @@
 ==Mouse PA28alpha-beta==
-<StructureSection load='5mx5' size='340' side='right' caption='[[5mx5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+<StructureSection load='5mx5' size='340' side='right'caption='[[5mx5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5mx5]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MX5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5mx5]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MX5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MX5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5msj|5msj]], [[5msk|5msk]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mx5 OCA], [http://pdbe.org/5mx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mx5 RCSB], [http://www.ebi.ac.uk/pdbsum/5mx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mx5 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mx5 OCA], [http://pdbe.org/5mx5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mx5 RCSB], [http://www.ebi.ac.uk/pdbsum/5mx5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mx5 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Groll, M]]
 [[Category: Huber, E M]]

5mx5

From Proteopedia

Revision as of 07:31, 19 August 2020

Mouse PA28alpha-beta

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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