1g71

From Proteopedia

(Difference between revisions)

Revision as of 07:07, 19 June 2019

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

Structural highlights

1g71 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	1dd9, 1dde, 1eqn
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PRIS_PYRFU] Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Primases are essential components of the DNA replication apparatus in every organism. They catalyze the synthesis of oligoribonucleotides on single-stranded DNA, which subsequently serve as primers for the replicative DNA polymerases. In contrast to bacterial primases, the archaeal enzymes are closely related to their eukaryotic counterparts. We have solved the crystal structure of the catalytic primase subunit from the hyperthermophilic archaeon Pyrococcus furiosus at 2.3 A resolution by multiwavelength anomalous dispersion methods. The structure shows a two-domain arrangement with a novel zinc knuckle motif located in the primase (prim) domain. In this first structure of a complete protein of the archaeal/eukaryotic primase family, the arrangement of the catalytically active residues resembles the active sites of various DNA polymerases that are unrelated in fold.

Crystal structure of a DNA-dependent RNA polymerase (DNA primase).,Augustin MA, Huber R, Kaiser JT Nat Struct Biol. 2001 Jan;8(1):57-61. PMID:11135672^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu L, Komori K, Ishino S, Bocquier AA, Cann IK, Kohda D, Ishino Y. The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus. J Biol Chem. 2001 Nov 30;276(48):45484-90. Epub 2001 Oct 2. PMID:11584001 doi:http://dx.doi.org/10.1074/jbc.M106391200
↑ Augustin MA, Huber R, Kaiser JT. Crystal structure of a DNA-dependent RNA polymerase (DNA primase). Nat Struct Biol. 2001 Jan;8(1):57-61. PMID:11135672 doi:10.1038/83060

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g71"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE==
-<StructureSection load='1g71' size='340' side='right' caption='[[1g71]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='1g71' size='340' side='right'caption='[[1g71]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1g71]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G71 FirstGlance]. <br>
@@ Line 14: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/1g71_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/1g71_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Augustin, M A]]
 [[Category: Huber, R]]

1g71

From Proteopedia

Revision as of 07:07, 19 June 2019

CRYSTAL STRUCTURE OF PYROCOCCUS FURIOSUS DNA PRIMASE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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