1hl3
From Proteopedia
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| - | [[Image:1hl3. | + | [[Image:1hl3.jpg|left|200px]]<br /><applet load="1hl3" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1hl3" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1hl3, resolution 3.1Å" /> | caption="1hl3, resolution 3.1Å" /> | ||
'''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE'''<br /> | '''CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HL3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1HL3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Nad Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HL3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transcription co-repression]] | [[Category: transcription co-repression]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:31:50 2007'' |
Revision as of 14:22, 18 December 2007
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CTBP/BARS IN TERNARY COMPLEX WITH NAD(H) AND PIDLSKK PEPTIDE
Overview
C-terminal-binding protein/brefeldin A-ADP ribosylated substrate, (CtBP/BARS) plays key roles in development and oncogenesis as a, transcription co-repressor, and in intracellular traffic as a promoter of, Golgi membrane fission. Co-repressor activity is regulated by NAD(H), binding to CtBP/BARS, while membrane fission is associated with its, acyl-CoA-dependent acyltransferase activity. Here, we report the crystal, structures of rat CtBP/BARS in a binary complex with NAD(H), and in a, ternary complex with a PIDLSKK peptide mimicking the consensus motif, (PXDLS) recognized in CtBP/BARS cellular partners. The structural data, show CtBP/BARS in a NAD(H)-bound dimeric form; the peptide binding maps, the recognition site for DNA-binding proteins and histone deacetylases to, an N-terminal region of the protein. The crystal structure together with, the site-directed mutagenesis data and binding experiments suggest a, rationale for the molecular mechanisms underlying the two fundamental, co-existing, but diverse, activities supported by CtBP/BARS in the nucleus, and in Golgi membranes.
About this Structure
1HL3 is a Single protein structure of sequence from Rattus norvegicus with NAD as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission., Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M, EMBO J. 2003 Jun 16;22(12):3122-30. PMID:12805226
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