2je4
From Proteopedia
(New page: 200px<br /> <applet load="2je4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2je4, resolution 1.07Å" /> '''ATOMIC-RESOLUTION C...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2JE4 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/ ]] with ACT, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JE4 OCA]]. | + | 2JE4 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]] with ACT, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JE4 OCA]]. |
==Reference== | ==Reference== | ||
Modular Total Chemical Synthesis of a Human Immunodeficiency Virus Type 1 Protease., Johnson EC, Malito E, Shen Y, Rich D, Tang WJ, Kent SB, J Am Chem Soc. 2007 Sep 19;129(37):11480-11490. Epub 2007 Aug 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705484 17705484] | Modular Total Chemical Synthesis of a Human Immunodeficiency Virus Type 1 Protease., Johnson EC, Malito E, Shen Y, Rich D, Tang WJ, Kent SB, J Am Chem Soc. 2007 Sep 19;129(37):11480-11490. Epub 2007 Aug 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17705484 17705484] | ||
| + | [[Category: Human immunodeficiency virus 1]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Johnson, E.C.B.]] | [[Category: Johnson, E.C.B.]] | ||
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[[Category: protease]] | [[Category: protease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:09:28 2007'' |
Revision as of 09:04, 30 October 2007
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ATOMIC-RESOLUTION CRYSTAL STRUCTURE OF CHEMICALLY-SYNTHESIZED HIV-1 PROTEASE IN COMPLEX WITH JG-365
Overview
As part of our ongoing studies of the human immunodeficiency virus type 1, (HIV-1) protease enzyme, we set out to develop a modular chemical, synthesis of the protein from multiple peptide segments. Our initial, attempts were frustrated by the insolubility of intermediate peptide, products. To overcome this problem, we designed a synthetic strategy, combining the solubility-enhancing properties of C-terminal (Arg)n tags, and the biological phenomenon of autoprocessing of the Gag-Pol polyprotein, that occurs during maturation of the HIV-1 virus in vivo. Synthesis of a, 119-residue peptide chain containing 10 residues of the reverse, transcriptase (RT) open reading frame plus an (Arg)10 tag at the, C-terminus was straightforward by native chemical ligation followed by, conversion of the Cys ... [(full description)]
About this Structure
2JE4 is a [Protein complex] structure of sequences from [Human immunodeficiency virus 1] with ACT, SO4 and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Modular Total Chemical Synthesis of a Human Immunodeficiency Virus Type 1 Protease., Johnson EC, Malito E, Shen Y, Rich D, Tang WJ, Kent SB, J Am Chem Soc. 2007 Sep 19;129(37):11480-11490. Epub 2007 Aug 18. PMID:17705484
Page seeded by OCA on Tue Oct 30 11:09:28 2007
