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1x22
From Proteopedia
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[[Image:1x22.gif|left|200px]] | [[Image:1x22.gif|left|200px]] | ||
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'''Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura''' | '''Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1X22 is a [[Single protein]] structure | + | 1X22 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X22 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Oizumi, Y.]] | [[Category: Oizumi, Y.]] | ||
[[Category: Yamakawa, M.]] | [[Category: Yamakawa, M.]] | ||
| - | [[Category: | + | [[Category: Antibacterial peptide]] |
| - | [[Category: | + | [[Category: Gene expression]] |
| - | [[Category: | + | [[Category: Insect immunity]] |
| - | [[Category: | + | [[Category: Moricin]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Purification]] |
| - | [[Category: | + | [[Category: Solution structure]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:25:48 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:25, 3 May 2008
Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura
Overview
An antibacterial peptide was isolated from a lepidopteran insect, Spodoptera litura. The molecular mass of this peptide was determined to be 4489.55 by matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) spectrometry. The peptide consists of 42 amino acids and the sequence has 69-98% identity to those of moricin-related peptides, antibacterial peptides from lepidopetran insects. Thus, the peptide was designated S. litura (Sl) moricin. Sl moricin showed a broad antibacterial spectrum against Gram-positive and negative bacteria. Sl moricin gene was inducible by bacterial injection and expressed tissue-specifically in the fat body and hemocytes. Furthermore, the solution structure of Sl moricin was determined by two-dimensional (2D) 1H-nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-simulated annealing calculation. The tertiary structure revealed a long alpha-helix containing eight turns along nearly the full length of the peptide like that of moricin, confirming that Sl moricin is a new moricin-like antibacterial peptide. These results suggest that moricin is present not only in B. mori but also in other lepidopteran insects forming a gene family.
About this Structure
1X22 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Isolation, gene expression and solution structure of a novel moricin analogue, antibacterial peptide from a lepidopteran insect, Spodoptera litura., Oizumi Y, Hemmi H, Minami M, Asaoka A, Yamakawa M, Biochim Biophys Acta. 2005 Aug 31;1752(1):83-92. PMID:16115804 Page seeded by OCA on Sat May 3 14:25:48 2008
