1x2g
From Proteopedia
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'''Crystal Structure of Lipate-Protein Ligase A from Escherichia coli''' | '''Crystal Structure of Lipate-Protein Ligase A from Escherichia coli''' | ||
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[[Category: Taniguchi, H.]] | [[Category: Taniguchi, H.]] | ||
[[Category: Toma, S.]] | [[Category: Toma, S.]] | ||
| - | [[Category: | + | [[Category: Lipoate-protein ligase]] |
| - | [[Category: | + | [[Category: Lipoic acid]] |
| - | [[Category: | + | [[Category: Post-translational modification]] |
| - | [[Category: | + | [[Category: Protein acylation]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:26:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:26, 3 May 2008
Crystal Structure of Lipate-Protein Ligase A from Escherichia coli
Overview
Lipoate-protein ligase A (LplA) catalyzes the formation of lipoyl-AMP from lipoate and ATP and then transfers the lipoyl moiety to a specific lysine residue on the acyltransferase subunit of alpha-ketoacid dehydrogenase complexes and on H-protein of the glycine cleavage system. The lypoyllysine arm plays a pivotal role in the complexes by shuttling the reaction intermediate and reducing equivalents between the active sites of the components of the complexes. We have determined the X-ray crystal structures of Escherichia coli LplA alone and in a complex with lipoic acid at 2.4 and 2.9 angstroms resolution, respectively. The structure of LplA consists of a large N-terminal domain and a small C-terminal domain. The structure identifies the substrate binding pocket at the interface between the two domains. Lipoic acid is bound in a hydrophobic cavity in the N-terminal domain through hydrophobic interactions and a weak hydrogen bond between carboxyl group of lipoic acid and the Ser-72 or Arg-140 residue of LplA. No large conformational change was observed in the main chain structure upon the binding of lipoic acid.
About this Structure
1X2G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site., Fujiwara K, Toma S, Okamura-Ikeda K, Motokawa Y, Nakagawa A, Taniguchi H, J Biol Chem. 2005 Sep 30;280(39):33645-51. Epub 2005 Jul 25. PMID:16043486 Page seeded by OCA on Sat May 3 14:26:32 2008
