1x2x
From Proteopedia
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'''NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings''' | '''NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X2X OCA]. | |
==Reference== | ==Reference== | ||
NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16972306 16972306] | NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16972306 16972306] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Alvarez-Salgado, F.]] | [[Category: Alvarez-Salgado, F.]] | ||
[[Category: Boulard, Y.]] | [[Category: Boulard, Y.]] | ||
[[Category: Desvaux, H.]] | [[Category: Desvaux, H.]] | ||
| - | [[Category: | + | [[Category: Deoxyribonucleic acid]] |
| - | [[Category: | + | [[Category: Liquid crystal]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Rdc]] |
| - | [[Category: | + | [[Category: Tandem gt mismatch]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:27:46 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:27, 3 May 2008
NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings
Overview
We have carried out a solution study of two non-labelled self-complementary DNA dodecamers d(GACTGTACAGTC)2 and d(GACTGTGCAGTC)2 by NMR, the second sequence composed of two G-T mismatches. Structures were determined using distances extracted from NOE effects alone or using both NOE and RDC constraints, measured in three different liquid crystalline media. We ensured that our data on the influence of the mesogen on the DNA structures, and the way in which the RDCs were incorporated as constraints in the protocol refinement, were consistent. We also tested the influence of different sets of RDCs and the best means of optimizing the calculation of D(a) and R. Resolution and accuracy of the ten best energy final structures were compared. The addition of a small set of RDC constraints significantly improves the final determined structures. We took advantage of the specificity of the RDC, i.e. it contains orientational information, and explored the global shape of the DNA duplexes; it was found that the duplexes do not have a large curvature. For the G-T base pair, we observed, in this particular sequence (tandem of G-T mismatches), a new pattern of base pairing, which involved the formation of a bifurcated hydrogen bond.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:16972306 Page seeded by OCA on Sat May 3 14:27:46 2008
